Construct: ORF TRCN0000481382
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012125.1_s317c1
- Derived from:
- ccsbBroadEn_10393
- DNA Barcode:
- GTGCCCAGTTAGCGTCGAGCGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GEMIN8P4 (492303)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481382
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | NM_001042479.2 | 46% | 45% | (many diffs) |
| 2 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | NM_001042480.2 | 46% | 45% | (many diffs) |
| 3 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | NM_017856.3 | 46% | 45% | (many diffs) |
| 4 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XM_017029617.2 | 46% | 45% | (many diffs) |
| 5 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XM_017029618.2 | 46% | 45% | (many diffs) |
| 6 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XM_005274555.3 | 32.4% | 31.7% | (many diffs) |
| 7 | human | 492303 | GEMIN8P4 | gem nuclear organelle assoc... | NR_002830.1 | 21.1% | 1_1008del;1372_1715del | |
| 8 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_001755705.2 | 19.8% | (many diffs) | |
| 9 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_001755704.2 | 19.5% | (many diffs) | |
| 10 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_001755702.1 | 18.6% | (many diffs) | |
| 11 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_001755703.1 | 16.2% | (many diffs) | |
| 12 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_950466.3 | 6.3% | (many diffs) | |
| 13 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_950464.3 | 6.2% | (many diffs) | |
| 14 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_950467.3 | 5.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 429
- ORF length:
- 363
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA GACCGAGTCA GATGCAGAGG TAGCATGTGA CCTGAGCAAT ATGGAAATCA 121 CCGAAGAGCT CCGCCAGTAC TTTGCAGAGA CAGAGAGGCA CAGAGAAGAA CTACTGCGGC 181 GGCAACAGCT GGATGCGGAG CGCCTGAACA GCTACGTGAA CGCTGAACAC GACCTGTACT 241 GCAACACCCG CCGGTCGGTA GAGGCCCCAG CTGAGAGGCC TGGTGAGCGG CGCCAGGCCG 301 AAATGAAGCG TTTGTACTGG GACAGCGCCG CCAAGATCCA GGCCATGGAG GCTAACGTGC 361 AGCTGAGCTT TGACAAGCAC TGTGATCCAA AGCAGCCTAA TTACTGGCCG GTCATCCCCC 421 CGAAGTTCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTGCCC AGTTAGCGTC GAGCGGCCAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt