Construct: ORF TRCN0000481393
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000138.1_s317c1
- Derived from:
- ccsbBroadEn_05502
- DNA Barcode:
- CTCAAGAACGCAGCAGTACCGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MACC1 (346389)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481393
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 346389 | MACC1 | MET transcriptional regulat... | NM_182762.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2625
- ORF length:
- 2556
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctaatcact gaaagaaaac attttcggtc aggaagaatt gcacaaagta 121 tgtctgaagc aaatttgatt gacatggaag ctggaaaact ctcaaaaagt tgcaatatta 181 cagaatgcca ggacccagac ttgcttcaca attggccgga tgctttcacc cttcgtggta 241 ataatgcttc caaagttgca aatccattct ggaatcaact gtctgcttct aacccatttt 301 tggatgacat aactcaacta agaaataaca ggaagagaaa taatatttcc atcttaaagg 361 aagatccttt tcttttctgt agagaaatag aaaatggaaa ttcttttgat tcctccggtg 421 atgaacttga tgtgcatcag ttacttaggc agacttcctc aagaaattct ggaagatcta 481 aaagtgtttc agaacttctg gacattttag acgacacagc acatgcccat cagagtatac 541 ataactctga ccagatccta ctacacgact tagagtggct taaaaatgat cgggaggctt 601 ataaaatggc ttggttaagt caacgccagc tggcccgctc ctgccttgat ttgaatacaa 661 ttagtcagag ccctggatgg gcccagacac aacttgcgga ggtcaccata gcttgcaaag 721 taaaccatca aggagggtca gtacaattac ctgaatcaga catcactgtt catgtgcccc 781 aaggtcatgt ggctgtggga gaattccaag aggtgtctct aagggctttc cttgatccgc 841 cacacatgct taaccatgat ctttcgtgca ctgtgagccc gttgttggaa atcatgttag 901 gcaacctcaa tacaatggaa gcccttttgc tggagatgaa aattggggct gaagtaagaa 961 aggatccttt cagccaagtc atgacagaaa tggtgtgttt acacagcttg ggtaaagaag 1021 gcccttttaa agttttaagc aactgctaca tttataaaga caccatccaa gtcaagctaa 1081 tcgacttgag tcaggtaatg tatctagtgg ttgctgcaca agctaaagct cttccgtcac 1141 cagctgccac catttgggat tatatccaca aaaccacctc aattggaatt tatggaccca 1201 aatatatcca tcccagtttt actgttgttt taacagtttg tggacacaat tatatgccag 1261 gacagcttac aatttctgat attaagaagg gtggaaaaaa catatctcca gttgtgtttc 1321 agctctgggg gaagcagtca tttttacttg acaagccaca agatttaagt atttctattt 1381 tttcctgtga tcctgatttt gaagtaaaga cagaaggaga aaggaaagaa attaaacaaa 1441 agcagttgga agcaggtgaa gtagttcatc aacaattttt attttcttta gttgagcaca 1501 gagagatgca cttgtttgat ttttgtgttc aagtggagcc tcccaatggt gaaccagttg 1561 cacagttctc tatcactact cctgatccaa ccccaaacct aaaaagactc tcgaatctgc 1621 caggctattt gcagaagaag gaggaaatca agtctgctcc tttatcacca aaaattcttg 1681 ttaaatatcc tacatttcaa gataaaacat tgaactttag caactatggg gtaaccctga 1741 aggcagtgct aagacaaagc aagattgatt acttccttga atatttcaaa ggggacacaa 1801 tagctctcct cggggaaggt aaggtaaaag ctattggtca gtccaaagtg aaagaatggt 1861 atgtaggagt cctcagaggt aagattggac ttgtacactg caaaaatgtc aaggtgattt 1921 caaaggagca agtaatgttt atgtcagata gtgtctttac aaccagaaat cttcttgaac 1981 agattgtcct gcctttaaaa aaattgactt atatctactc agttgtatta accttggtgt 2041 cagaaaaagt ttatgattgg aaagttttag ctgatgtcct gggttactca catctgtccc 2101 tggaagattt tgatcaaatt caagcagaca aagaatcaga gaaagtttct tatgttataa 2161 agaagttaaa ggaagattgC CACACAGAGA GAAATACAAG GAAGTTTCTG TATGAACTTA 2221 TTGTGGCTCT TCTGAAAATG GATTGCCAAG AGTTAGTCGC ACGTCTCATC CAAGAAGCTG 2281 CTGTTCTGAC TTCAGCTGTC AAGCTTGGAA AAGGCTGGAG GGAACTAGCT GAAAAGTTAG 2341 TACGACTCAC AAAGCAACAA ATGGAGGCAT ATGAAATTCC TCATCGAGGA AACACTGGAG 2401 ATGTTGCTGT TGAGATGATG TGGAAACCTG CCTATGATTT TCTGTATACC TGGAGTGCTC 2461 ACTATGGAAA TAACTACAGA GATGTGTTAC AAGACCTTCA GTCAGCTTTG GACAGAATGA 2521 AAAACCCTGT GACTAAACAC TGGAGAGAAT TAACTGGAGT TTTAATACTA GTAAATTCTT 2581 TGGAGGTTTT GAGAGTAACT GCATTCTCCA CTTCTGAGGA AGTATTGCCA ACTTTCTTGT 2641 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2701 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2761 GAAAGGACGA CTCAAGAACG CAGCAGTACC GATGACGCGT TAAGTCgaca atcaacctct 2821 ggattacaaa atttgtgaaa gatt