Construct: ORF TRCN0000481403

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014423.1_s317c1
Derived from:
ccsbBroadEn_01292
DNA Barcode:
CTTCTGCCCGCATGAGTCTAAACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EIF2AK2 (5610)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481403
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5610 EIF2AK2 eukaryotic translation init... NM_001135651.3 100% 100%
2 human 5610 EIF2AK2 eukaryotic translation init... NM_002759.3 100% 100%
3 human 5610 EIF2AK2 eukaryotic translation init... XM_011532987.2 100% 100%
4 human 5610 EIF2AK2 eukaryotic translation init... NM_001135652.2 92.5% 92.5% 785_786ins123
5 human 5610 EIF2AK2 eukaryotic translation init... XM_017004503.1 92.1% 92.1% 1246_1247ins129
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1722
ORF length:
1653
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggctggtgat ctttcagcag gtttcttcat ggaggaactt aatacatacc
121gtcagaagca gggagtagta cttaaatatc aagaactgcc taattcagga cctccacatg
181ataggaggtt tacatttcaa gttataatag atggaagaga atttccagaa ggtgaaggta
241gatcaaagaa ggaagcaaaa aatgccgcag ccaaattagc tgttgagata cttaataagg
301aaaagaaggc agttagtcct ttattattga caacaacgaa ttcttcagaa ggattatcca
361tggggaatta cataggcctt atcaatagaa ttgcccagaa gaaaagacta actgtaaatt
421atgaacagtg tgcatcgggg gtgcatgggc cagaaggatt tcattataaa tgcaaaatgg
481gacagaaaga atatagtatt ggtacaggtt ctactaaaca ggaagcaaaa caattggccg
541ctaaacttgc atatcttcag atattatcag aagaaacctc agtgaaatct gactacctgt
601cctctggttc ttttgctact acgtgtgagt cccaaagcaa ctctttagtg accagcacac
661tcgcttctga atcatcatct gaaggtgact tctcagcaga tacatcagag ataaattcta
721acagtgacag tttaaacagt tcttcgttgc ttatgaatgg tctcagaaat aatcaaagga
781aggcaaaaag atctttggca cccagatttg accttcctga catgaaagaa acaaagtata
841ctgtggacaa gaggtttggc atggatttta aagaaataga attaattggc tcaggtggat
901ttggccaagt tttcaaagca aaacacagaa ttgacggaaa gacttacgtt attaaacgtg
961ttaaatataa taacgagaag gcggagcgtg aagtaaaagc attggcaaaa cttgatcatg
1021taaatattgt tcactacaat ggctgttggg atggatttga ttatgatcct gagaccagtg
1081atgattctct tgagagcagt gattatgatc ctgagaacag caaaaatagt tcaaggtcaa
1141agactaagtg ccttttcatc caaatggaat tctgtgataa agggaccttg gaacaatgga
1201ttgaaaaaag aagaggcgag aaactagaca aagttttggc tttggaactc tttgaacaaa
1261taacaaaagg ggtggattat atacattcaa aaaaaTTAAT TCATAGAGAT CTTAAGCCAA
1321GTAATATATT CTTAGTAGAT ACAAAACAAG TAAAGATTGG AGACTTTGGA CTTGTAACAT
1381CTCTGAAAAA TGATGGAAAG CGAACAAGGA GTAAGGGAAC TTTGCGATAC ATGAGCCCAG
1441AACAGATTTC TTCGCAAGAC TATGGAAAGG AAGTGGACCT CTACGCTTTG GGGCTAATTC
1501TTGCTGAACT TCTTCATGTA TGTGACACTG CTTTTGAAAC ATCAAAGTTT TTCACAGACC
1561TACGGGATGG CATCATCTCA GATATATTTG ATAAAAAAGA AAAAACTCTT CTACAGAAAT
1621TACTCTCAAA GAAACCTGAG GATCGACCTA ACACATCTGA AATACTAAGG ACCTTGACTG
1681TGTGGAAGAA AAGCCCAGAG AAAAATGAAC GACACACATG TTTGCCAACT TTCTTGTACA
1741AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1801AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1861AGGACGACTT CTGCCCGCAT GAGTCTAAAC GACGCGTTAA GTCgacaatc aacctctgga
1921ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

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