Construct: ORF TRCN0000481406
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008589.2_s317c1
- Derived from:
- ccsbBroadEn_14539
- DNA Barcode:
- TTGATACCTCTGGACACCCCTTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH1A3 (220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481406
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220 | ALDH1A3 | aldehyde dehydrogenase 1 fa... | NM_001293815.2 | 13.6% | 10.2% | (many diffs) |
| 2 | human | 220 | ALDH1A3 | aldehyde dehydrogenase 1 fa... | NM_000693.4 | 10.7% | 8.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 247
- ORF end:
- 430
- ORF length:
- 183
- Sequence:
-
1 GGAGTACGTC GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG 61 AGTGGGTGGA GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG 121 CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT 181 TTCTTCCATT TCAGGTGTCG TGAGGCTAGC ATCGATTGAT CAACAAGTTT GTACAAAAAA 241 GTTGGCATGG CCACCGCTAA CGGGGCCGTG GAAAACGGGC AGCCGGACAG GAAGCCGCCG 301 GCCCTGCCGC GCCCCATCCG CAACCTGGAG GTCAAGTTCA CCAAGGTGAG GCGGGCGCCC 361 CTCCCACCCG ACGGCCGCGG GCCCCTGCGC TGGGCAGCCA GACTCGGGGC GGCGGGACTC 421 GGCGCTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 481 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 541 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTGATAC CTCTGGACAC CCCTTTTACG 601 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt