Construct: ORF TRCN0000481412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003087.1_s317c1
- Derived from:
- ccsbBroadEn_01418
- DNA Barcode:
- ACTTATTTCTAGCCTTCCGGCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL7 (6129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6129 | RPL7 | ribosomal protein L7 | NM_000971.4 | 100% | 100% | |
| 2 | human | 6129 | RPL7 | ribosomal protein L7 | NM_001363737.1 | 83.8% | 83.8% | 0_1ins120 |
| 3 | mouse | 19989 | Rpl7 | ribosomal protein L7 | NM_011291.5 | 81.7% | 88.8% | (many diffs) |
| 4 | mouse | 620678 | Gm12174 | predicted gene 12174 | XR_141051.5 | 72.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 810
- ORF length:
- 744
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gggtgtagaa gagaagaaga aggaggttcc tgctgtgcca gaaaccctta 121 agaaaaagcg aaggaatttc gcagagctga agatcaagcg cctgagaaag aagtttgccc 181 aaaagatgct tcgaaaggca aggaggaagc ttatctatga aaaagcaaag cactatcaca 241 aggaatatag gcagatgtac agaactgaaa ttcgaatggc gaggatggca agaaaagctg 301 gcaacttcta tgtacctgca gaacccaaat tggcgtttgt catcagaatc agaggtatca 361 atggagtgag cccaaaggtt cgaaaggtgt tgcagcttct tcgccttcgt caaatcttca 421 atggaaCCTT TGTGAAGCTC AACAAGGCTT CGATTAACAT GCTGAGGATT GTAGAGCCAT 481 ATATTGCATG GGGGTACCCC AATCTGAAGT CAGTAAATGA ACTAATCTAC AAGCGTGGTT 541 ATGGCAAAAT CAATAAGAAG CGAATTGCTT TGACAGATAA CGCTTTGATT GCTCGATCTC 601 TTGGTAAATA CGGCATCATC TGCATGGAGG ATTTGATTCA TGAGATCTAT ACTGTTGGAA 661 AACGCTTCAA AGAGGCAAAT AACTTCCTGT GGCCCTTCAA ATTGTCTTCT CCACGAGGTG 721 GAATGAAGAA AAAGACCACC CATTTTGTAG AAGGTGGAGA TGCTGGCAAC AGGGAGGACC 781 AGATCAACAG GCTTATTAGA AGAATGAACT ACCCAACTTT CTTGTACAAA GTGGTTGATA 841 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 901 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACTTA 961 TTTCTAGCCT TCCGGCGTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1021 tgaaagatt