Construct: ORF TRCN0000481431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005947.1_s317c1
- Derived from:
- ccsbBroadEn_05176
- DNA Barcode:
- AAGATGTTGTCGGCGTGGTTCGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MLKL (197259)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 197259 | MLKL | mixed lineage kinase domain... | NM_001142497.3 | 100% | 100% | |
| 2 | human | 197259 | MLKL | mixed lineage kinase domain... | XM_011522936.3 | 76.2% | 62.8% | (many diffs) |
| 3 | human | 197259 | MLKL | mixed lineage kinase domain... | NM_152649.4 | 52.4% | 52.8% | 536_1190del;1239_1240ins31 |
| 4 | human | 197259 | MLKL | mixed lineage kinase domain... | XM_005255834.4 | 52.4% | 52.8% | 536_1190del;1239_1240ins31 |
| 5 | human | 197259 | MLKL | mixed lineage kinase domain... | XR_001751863.2 | 33.1% | (many diffs) | |
| 6 | human | 197259 | MLKL | mixed lineage kinase domain... | XR_001751862.2 | 31.9% | (many diffs) | |
| 7 | human | 197259 | MLKL | mixed lineage kinase domain... | XR_429715.4 | 31.4% | 1_412del;948_1602del;1857_2508del | |
| 8 | human | 197259 | MLKL | mixed lineage kinase domain... | XR_001751861.2 | 31.3% | (many diffs) | |
| 9 | human | 197259 | MLKL | mixed lineage kinase domain... | XR_001751860.2 | 31.2% | (many diffs) | |
| 10 | human | 197259 | MLKL | mixed lineage kinase domain... | XR_001751859.2 | 30% | (many diffs) | |
| 11 | human | 197259 | MLKL | mixed lineage kinase domain... | XR_001751858.2 | 29.7% | (many diffs) | |
| 12 | human | 197259 | MLKL | mixed lineage kinase domain... | XR_002957791.1 | 28.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 855
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaatttgaag catattatca cccttggcca ggtcatccac aaacggtgtg 121 aagagatgaa atactgcaag aaacagtgcc ggcgcctggg ccaccgcgtc ctcggcctga 181 tcaagcctct ggagatgctc caggaccaag gaaagaggag cgtgccctct gagaagttaa 241 ccacagccat gaaccgcttc aaggctgccc tggaggaggc taatggggag atagaaaagt 301 tcagcaatag atccaatatc tgcaggtttc TAACAGCAAG CCAGGACAAA ATACTCTTCA 361 AGGACGTGAA CAGGAAGCTG AGTGATGTCT GGAAGGAGCT CTCGCTGTTA CTTCAGGTTG 421 AGCAACGCAT GCCTGTTTCA CCCATAAGCC AAGGAGCGTC CTGGGCACAG GAAGATCAGC 481 AGGATGCAGA CGAAGACAGG CGAGCTTTCC AGATGCTAAG AAGAGATAAT GAAAAAATAG 541 AAGCTTCACT GAGACGATTA GAAATCAACA TGAAAGAAAT CAAGGAAACT TTGAGGCAGT 601 CTTTGGAATC GTCCTCTGGG AAATCGCCAC TGGAGATATC CCGTTTCAAG GTGAAGAATG 661 TGAAGACTGG CTCAGCCAGT GGCTGTAATT CTGAGAAGAT CCGCAAGCTG GTGGCTGTGA 721 AGCGGCAGCA GGAGCCACTG GGTGAAGACT GCCCTTCAGA GCTGCGGGAG ATCATTGATG 781 AGTGCCGGGC CCATGATCCC TCTGTGCGGC CCTCTGTGGA TGAAATCTTA AAGAAACTCT 841 CCACCTTTTC TAAGTACCCA ACTTCCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 901 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 961 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAGATGTTGT CGGCGTGGTT 1021 CGCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt