Construct: ORF TRCN0000481432
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005294.1_s317c1
- Derived from:
- ccsbBroadEn_14301
- DNA Barcode:
- TGCGAGCCACCGCGAAGAGGTGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EMILIN2 (84034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481432
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84034 | EMILIN2 | elastin microfibril interfa... | NM_032048.3 | 99.9% | 54.2% | 414C>T;1673_1674insG;2364G>A |
| 2 | human | 84034 | EMILIN2 | elastin microfibril interfa... | XM_017026038.2 | 77.9% | 68.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1893
- ORF length:
- 1824
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggcagccc agacggccct ggccccgcgt gccctggcgc tgggcgctgg 121 cgctgctggc cctggttggc gcggggctgt gccacgccgg cccgcagccc gggtatcccg 181 cgcggcccag cgccaggaac aagaactggt gcgcctacat cgtgaacaag aatgtgagct 241 gctccgtgct ggagggaagt gagagtttta ttcaggctca gtacaactgt gcctggaacc 301 agatgccctg tccgtcggcg ctggtgtatc gagtgaactt cagacctaga tatgtcacta 361 ggtataagac agtgacacag ttggaatgga ggtgctgtcc tggctttaga gggggagatt 421 gccaagaagg tcccaaagac cccgtgaaga ccctccgccc cacgccggct cggcctcgaa 481 atagcttgaa gaaagccaca gataatgaac ccagccaatt ctcagagccc aggaagactt 541 tgtccccaac tggtacagca caaccaagct ggggggtaga tccaaaagag gggcctcagg 601 aacttcagga aaagaagata caggtgctag aggagaaggt tcttcgactc acaaggacgg 661 ttcttgacct ccagtcttcc cttgctggag tgagtgaaaa tctcaaacat gccactcagg 721 atgatgccag tagaacacgg gcaccagggc tcagcagcca gcaccccaag cctgacacca 781 ctgttagtgg agacacagaa acgggccaga gtcctggtgt cttcaacact aaggaatctg 841 gcatgaagga catcaagtct gaattggctg aagtcaaaga tactctaaag aacaaaagtg 901 acaagctgga agagctggat ggaaaagtga agggctacga agggcagctc agacagctcc 961 aggaagcagc tcagggcccg acggtgacca tgacaaccaa cgaactctac caagcctatg 1021 tggacagtaa gatcgacgcc ctgagagagg agctcatgga gggcatggac agaaagctgg 1081 ctgacctgaa aaactcatgt gagtacaagc tcactggcct ccagcagcag tgtgatgact 1141 atgggagcag ctacctggga gtgatagagc tcatagggga gaaggaaaca agcctgagaa 1201 aagaaataaa taacctccga gcccggctac aggagccttc agcccaggca aattgctgcg 1261 acagtgaaaa gaatggtgac attggtcaac agatcaagac attggaccag aaaatcgaga 1321 gagttgctga agccaccaga atgctgaatg gaagactgga caatgagttt gaccgcctta 1381 tagttccaga gccagatgtg gattttgatg caaaatggaa tgaactcgat gcaaggatca 1441 atgtgacgga gaagaacgct gaagaacatt gcttttacat tgaggaaacc cttcggggcg 1501 ccattaatgg agaggtgggt gacttgaagc agcttgttga tcagaaaata cagtctctgg 1561 aagaccgtct ggggagcgtt ctcctacaga tgaccaataa cactggtgca gagctcagtc 1621 ccccaggggc agcagccctg ccaggagtgt cagggtcagg agatgaacgg gtcatgatgg 1681 aattaaacca cctgaaggac aaagttcaag ttgttgaaga catttgcctg ctgaacatcc 1741 aggggaaagc ctcatgggat ggaaggtgcc ttgccaaaca gggaagaccg cgcagtacgc 1801 gacagcctgc accttttgaa atctctcaac gacacgatgc acaggaagtt tcaagaaacc 1861 gaacaaacca tccagaaact tcaacaggat tttagttttc tttattctca attaaaccac 1921 acagaaaatg atgtgactca tcttcaaaag gaaatgagca attgtagagc aggtgaaaac 1981 gctggcatgg gtaggttcac taaggtgggt gagcaagaaa ggacagtgga caccctgccg 2041 tccccccagc accccgtggc tcattgctgc agtcagctgg aggagaggtg gcagaggttg 2101 cagagccagg tcatctcgga gctggatgct tgtaaggaat gcacgcaggg ggtccagagg 2161 gaggtctcca tggtggaggg cagggtgtct catatggaga aaacttgcag caagctggac 2221 tctatctcag gaaatcttca gaggatcaag gaggggctca acaagcatgt cagcagcctg 2281 tggaactgtg tcaggcagat gaacggaacg ctcaggtcgc attccagaga catttctggc 2341 ctgaagaatt cagtccagca gttctacagc cacgtcttcc agatttctac tgatttgcaa 2401 gatctggtca aatttcagcc atcagcaaag gcaccctcgc ccccgccgcc cgcagaggcc 2461 ccgaaggagc cgctgcagcc cgagcccgcc ccgccgaggc ccagcggccc cgcaaccgca 2521 gaggaccctg ggcgacggcc cgtcctgccc cagcggcccc ccgaggagag gccgccccag 2581 ccgccaggct ccaccggggt catcgcggag acgggccagg ccgggccccc cgcaggcgca 2641 ggcgtgtctg ggcggggtct gccgcggggc gtggacggcc agaccgggag cggcaccgtc 2701 cccggcgcag aaggcttcgc gggcgcacca ggatacccga agtcacctcc tgtagcttcc 2761 ccaggagctc cggtgccttc tctggtgtct ttttctgcgg ggctcaccca gaagcctttc 2821 cccagtgatg ggggcgttgt cctctttaac aaagtgctgg tgaacgacgg gGATGTTTAC 2881 AACCCCAGCA CCGGGGTCTT CACGGCTCCT TATGATGGGC GCTACCTGAT CACGGCCACC 2941 CTCACCCCCG AGAGAGACGC CTACGTGGAA GCAGTGCTGT CGGTCTCCAA CGCCAGCGTG 3001 GCCCAGCTGC ATACCGCTGG GTACAGGAGA GAGTTCCTGG AATACCACCG CCCTCCAGGA 3061 GCTTTGCATA CCTGCGGGGG CCCGGGGGCA TTCCACCTCA TCGTGCACCT GAAGGCGGGA 3121 GATGCAGTCA ACGTCGTGGT GACTGGGGGC AAGCTGGCTC ACACAGACTT TGATGAAATG 3181 TACTCCACAT TTAGTGGGGT TTTCTTATAT CCTTTCCTTT CCCACCTCTT GCCAACTTTC 3241 TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT 3301 ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 3361 TGTGGAAAGG ACGATGCGAG CCACCGCGAA GAGGTGTGAC GCGTTAAGTC gacaatcaac 3421 ctctggatta caaaatttgt gaaagatt