Construct: ORF TRCN0000481433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004848.1_s317c1
- Derived from:
- ccsbBroadEn_00006
- DNA Barcode:
- ATAGGATTAATGTACGTTCGTCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACADM (34)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 34 | ACADM | acyl-CoA dehydrogenase medi... | NM_000016.5 | 100% | 100% | |
| 2 | human | 34 | ACADM | acyl-CoA dehydrogenase medi... | NM_001127328.2 | 99% | 99% | 30_41del |
| 3 | human | 34 | ACADM | acyl-CoA dehydrogenase medi... | NM_001286043.1 | 92.7% | 92.7% | 285_383del |
| 4 | human | 34 | ACADM | acyl-CoA dehydrogenase medi... | NM_001286042.1 | 91.1% | 90.7% | (many diffs) |
| 5 | human | 34 | ACADM | acyl-CoA dehydrogenase medi... | NM_001286044.1 | 55.1% | 55.1% | 0_1ins567 |
| 6 | mouse | 11364 | Acadm | acyl-Coenzyme A dehydrogena... | NM_007382.5 | 82.6% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcggggttc gggcgatgct gcagggtcct gagaagtatt tctcgttttc 121 attggagatc acagcataca aaagccaatc gacaacgtga accaggatta ggatttagtt 181 ttgagttcac cgaacagcag aaagaatttc aagctactgc tcgtaaattt gccagagagg 241 aaatcatccc agtggctgca gaatatgata aaactggtga atatccagtc cccctaatta 301 gaagagcctg ggaacttggt ttaatgaaca cacacattcc agagaactgt ggaggtcttg 361 gacttggaac ttttgatgct tgtttaatta gtgaagaatt ggcttatgga tgtacagggg 421 ttcagactgc tattgaagga aattctttgg ggcaaatgcc tattattatt gctggaaatg 481 atcaacaaaa gaagaagtat ttggggagaa tgactgagga gccattgatg tgtgcttatt 541 gtgtaacaga acctggagca ggctctgatg tagctggtat aaagaccaaa gcagaaaaga 601 aaggagatga gtatattatt aatggtcaga agatgtggat aaccaacgga ggaaaagcta 661 attggtattt tttattggca cgttctgatc cagatcctaa agctcctgct aataaagcct 721 ttactggatt cattgtggaa gcagataccc caggaattca gattgggaga aaggaattaa 781 acatgggcca gcgatgttca gatactagag gaattgtctt cgaagatgtg aaagtgccta 841 aagaaaatgt tttaattggt gacggagctg gtttcaaagt tgcaatggga gcttttgata 901 aaaCCAGACC TGTAGTAGCT GCTGGTGCTG TTGGATTAGC ACAAAGAGCT TTGGATGAAG 961 CTACCAAGTA TGCCCTGGAA AGGAAAACTT TCGGAAAGCT ACTTGTAGAG CACCAAGCAA 1021 TATCATTTAT GCTGGCTGAA ATGGCAATGA AAGTTGAACT AGCTAGAATG AGTTACCAGA 1081 GAGCAGCTTG GGAGGTTGAT TCTGGTCGTC GAAATACCTA TTATGCTTCT ATTGCAAAGG 1141 CATTTGCTGG AGATATTGCA AATCAGTTAG CTACTGATGC TGTGCAGATA CTTGGAGGCA 1201 ATGGATTTAA TACAGAATAT CCTGTAGAAA AACTAATGAG GGATGCCAAA ATCTATCAGA 1261 TTTATGAAGG TACTTCACAA ATTCAAAGAC TTATTGTAGC CCGTGAACAC ATTGACAAGT 1321 ACAAAAATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATAGGA TTAATGTACG TTCGTCCGAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt