Construct: ORF TRCN0000481476
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017568.1_s317c1
- Derived from:
- ccsbBroadEn_02336
- DNA Barcode:
- CGGCTGACGAGCCGCAATGACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPAR6 (10161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481476
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | NM_001162497.2 | 100% | 100% | |
| 2 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | NM_001162498.2 | 100% | 100% | |
| 3 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | NM_005767.6 | 100% | 100% | |
| 4 | human | 10161 | LPAR6 | lysophosphatidic acid recep... | XM_024449302.1 | 100% | 100% | |
| 5 | mouse | 67168 | Lpar6 | lysophosphatidic acid recep... | NM_175116.4 | 87.4% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1101
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtaagcgtt aacagctccc actgcttcta taatgactcc tttaagtaca 121 ctttgtatgg gtgcatgttc agcatggtgt ttgtgcttgg gttaatatcc aattgtgttg 181 ccatatacat tttcatctgc gtcctcaaag tccgaaatga aactacaact tacatgatta 241 acttggcaat gtcagacttg ctttttgttt ttactttacc cttcaggatt ttttacttca 301 caacacggaa ttggccattt ggagatttac tttgtaagat ttctgtgatg ctgttttata 361 ccaacatgta cggaagcatt ctgttcttaa cctgtattag tgtagatcga tttctggcaa 421 ttgtctaccc atttaagtca aagactctaa gaaccaaaag aaatgcaaag attgtttgca 481 ctggcgtgtg gttaactgtg atcggaggaa gtgcacccgc cgtttttgtt cagtctaccc 541 actctcaggg taacaatgcc tcagaagcct gctttgaaaa ttttccagaa gccacatgga 601 aaacatatct ctcaaggatt gtaattttca tcgaaatagt gggatttttt attcctctaa 661 ttttaaatgt aacttgttcT AGTATGGTGC TAAAAACTTT AACCAAACCT GTTACATTAA 721 GTAGAAGCAA AATAAACAAA ACTAAGGTTT TAAAAATGAT TTTTGTACAT TTGATCATAT 781 TCTGTTTCTG TTTTGTTCCT TACAATATCA ATCTTATTTT ATATTCTCTT GTGAGAACAC 841 AAACATTTGT TAATTGCTCA GTAGTGGCAG CAGTAAGGAC AATGTACCCA ATCACTCTCT 901 GTATTGCTGT TTCCAACTGT TGTTTTGACC CTATAGTTTA CTACTTTACA TCGGACACAA 961 TTCAGAATTC AATAAAAATG AAAAACTGGT CTGTCAGGAG AAGTGACTTC AGATTCTCTG 1021 AAGTTCATGG TGCAGAGAAT TTTATTCAGC ATAACCTACA GACCTTAAAA AGTAAGATAT 1081 TTGACAATGA ATCTGCTGCC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGGC TGACGAGCCG 1261 CAATGACCCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt