Construct: ORF TRCN0000481491
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007618.1_s317c1
- Derived from:
- ccsbBroadEn_11236
- DNA Barcode:
- GACTCCGTCAACCTGCTACCACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF148 (7707)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481491
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348434.2 | 16% | 14.5% | (many diffs) |
| 2 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348424.1 | 15.2% | 13.7% | (many diffs) |
| 3 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348425.2 | 15.2% | 13.7% | (many diffs) |
| 4 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348426.2 | 15.2% | 13.7% | (many diffs) |
| 5 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348427.2 | 15.2% | 13.7% | (many diffs) |
| 6 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348428.2 | 15.2% | 13.7% | (many diffs) |
| 7 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348429.2 | 15.2% | 13.7% | (many diffs) |
| 8 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348430.2 | 15.2% | 13.7% | (many diffs) |
| 9 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348431.2 | 15.2% | 13.7% | (many diffs) |
| 10 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348432.2 | 15.2% | 13.7% | (many diffs) |
| 11 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_001348433.2 | 15.2% | 13.7% | (many diffs) |
| 12 | human | 7707 | ZNF148 | zinc finger protein 148 | NM_021964.2 | 15.2% | 13.7% | (many diffs) |
| 13 | mouse | 22661 | Zfp148 | zinc finger protein 148 | XM_006522104.3 | 22.4% | 20.9% | (many diffs) |
| 14 | mouse | 22661 | Zfp148 | zinc finger protein 148 | XM_006522103.3 | 15.5% | 14.4% | (many diffs) |
| 15 | mouse | 22661 | Zfp148 | zinc finger protein 148 | NM_011749.4 | 14.7% | 13.6% | (many diffs) |
| 16 | mouse | 22661 | Zfp148 | zinc finger protein 148 | XM_006522102.3 | 14.7% | 13.6% | (many diffs) |
| 17 | mouse | 22661 | Zfp148 | zinc finger protein 148 | XM_017316980.1 | 14.7% | 13.6% | (many diffs) |
| 18 | mouse | 22661 | Zfp148 | zinc finger protein 148 | XM_006522098.3 | 14.6% | 13.5% | (many diffs) |
| 19 | mouse | 22661 | Zfp148 | zinc finger protein 148 | XM_006522099.3 | 14.6% | 13.5% | (many diffs) |
| 20 | mouse | 22661 | Zfp148 | zinc finger protein 148 | XM_006522100.3 | 14.6% | 13.5% | (many diffs) |
| 21 | mouse | 22661 | Zfp148 | zinc finger protein 148 | XM_006522101.3 | 14.6% | 13.5% | (many diffs) |
| 22 | mouse | 22661 | Zfp148 | zinc finger protein 148 | XM_011245888.2 | 14.6% | 13.5% | (many diffs) |
| 23 | mouse | 22171 | Tyms | thymidylate synthase | XR_001784663.1 | 9.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCG TTTCATACAG AAGTACCTGC TTCAGAGACA TGAGAAGATT CATACTGGTG 121 AAAAACCATT TCGCTGTGAT GAATGTGGTG ATGAGACAAA CCATGCTTCT GCCACATCAA 181 CACAGGACTT TCTGGATCAA GTGACTTCTC AGAAGAAAGC TGAGGCCCAG CCTGTCCACC 241 AAGCTTACCA AATGAGCTCC TTTGAACAGC CCTTCCGTGC TCCCTATCAT GGATCAAGAG 301 CTGGAATAGC TACTCAATTT AGCACTGCCA ATGGACAGGT GAACCTTCGG GGACCAGGGA 361 CAAGTGCTGA ATTTTCAGAA TTTCCCTTGG TGAATGTAAA TGATAATAGA GCTGGGATGA 421 CATCTTCACC TGATGCCACA ACTGGCCAGA CTTTTGGCTA CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGAGACTCC GTCAACCTGC TACCACTAAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt