Construct: ORF TRCN0000481503
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015420.1_s317c1
- Derived from:
- ccsbBroadEn_09617
- DNA Barcode:
- CGTGCTCCCGCGCTCGGATTTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R36 (145376)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481503
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145376 | PPP1R36 | protein phosphatase 1 regul... | NM_172365.3 | 99.9% | 99.7% | 248C>T |
| 2 | human | 145376 | PPP1R36 | protein phosphatase 1 regul... | XM_005267354.4 | 94.6% | 95.7% | (many diffs) |
| 3 | human | 145376 | PPP1R36 | protein phosphatase 1 regul... | XM_005267355.4 | 89.4% | 89% | 0_1ins132;2T>G;116C>T |
| 4 | human | 145376 | PPP1R36 | protein phosphatase 1 regul... | XM_011536468.3 | 89.2% | 89% | 248C>T;533_534ins135 |
| 5 | human | 145376 | PPP1R36 | protein phosphatase 1 regul... | XM_024449485.1 | 57.8% | 57.8% | 0_1ins399;134_135ins135 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1335
- ORF length:
- 1266
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtaccgggtg cccgagtttt atgcgaggag gaagcggtta ggtgggcaga 121 ccccttactt gatggatcag ttgggattac gattagggat gtggtactgg aaagatgaaa 181 ccagaactct tgaattcaga aggtttgccg cagaagattc tgtccagtgg ctcctgaaac 241 atcaccctca ttttacacct gcagcagaag tcaaggaaaa aggaaagaaa ggcaaagcag 301 ttcactttgc agaaattgat ggtccagctt cagacaggtt gacagataaa agacttgctg 361 caaaagatga taagtcagct aaagctgtag agaaacgagg tcaacagggc accattacac 421 tggatgatgt taaatttgtt actttgcttt tgctacaaga tactgagatg cagcggatct 481 gttcttttac aacatttatg aggaataaga atcttgataa tttcctcatg gcattattgt 541 actacttatc ccattacttg gaaaaaaact cactggaaaa gaaacccaaa agctatatgg 601 taggccttgt agagaaaaaa gaaatggaat tggttttaag tgaattagaa gcagcacaga 661 ggtacttggc gcagaagtac tgtatccttg tgctgggctt ggccgtgccg gataagcatc 721 acatgtgctg tggaaaggag aaaatatcag atacacagaa agactggaag ttctttgagt 781 ccttttatac tttctgtaca tatgtggctt ggattgtctt ccgacgtcaa cacttgacag 841 agattgaaga agaagtaggg agactctttc gtaccaatat gttcaacatT CCTCGCAGGA 901 GGCGTGAAGA TGAGGAATCA GGAGGGGAAA AGAAACGCAT GACTTTTGTT CAGTTCAGGA 961 GAATGATGGC AAAACGCCCA GCAATTAAAA AAGCTATCAA CATGCGTTCT CCAGTCATGT 1021 CTACTCTGCT GCCATCTCTC AGAGAAAAAG CTCAAAACGT CTTTGAGAAA AAGTATCATC 1081 AAGTAGACGT CAGATTCCCA GCCGAGATGC AAAAGCATGT GGGAACTCTG GACTCTGTGC 1141 CCATGCCGGT AGTTGGCATC TTGGGGGAGC CTCGATGTCT ATTCAACCCA CATACGCTTC 1201 ACCCCCTTGA TCCAGAAGAA AACACAAAAT CATTTGGGAG ATATCCTTCC TTGATGGAAA 1261 ACAATAACAT GAGGATTCAG GATACACTGG ACTTGGTCAT GAAAACACTG TCCTCTCATA 1321 CATCATGCCC TAAGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1381 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1441 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGTGCTCCCG CGCTCGGATT 1501 TTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt