Construct: ORF TRCN0000481512
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008792.1_s317c1
- Derived from:
- ccsbBroadEn_15661
- DNA Barcode:
- GCCGAGAACACCGGAACCAAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX21 (9188)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481512
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9188 | DDX21 | DExD-box helicase 21 | NM_001256910.1 | 100% | 100% | |
| 2 | human | 9188 | DDX21 | DExD-box helicase 21 | XM_017016910.2 | 92.4% | 92.4% | 1180_1181ins162 |
| 3 | human | 9188 | DDX21 | DExD-box helicase 21 | NM_004728.4 | 91.3% | 91.3% | 1_204del |
| 4 | human | 9188 | DDX21 | DExD-box helicase 21 | XM_011540336.1 | 84.4% | 84.4% | 1_204del;1384_1385ins162 |
| 5 | mouse | 56200 | Ddx21 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_019553.2 | 70.6% | 71.9% | (many diffs) |
| 6 | mouse | 56200 | Ddx21 | DEAD (Asp-Glu-Ala-Asp) box ... | XR_001779560.1 | 35.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2211
- ORF length:
- 2145
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttctcctaaa tccaaaaagg caaaaaagaa agaggagcca tctcaaaatg 121 acatttctcc taaaaccaaa agtttgagaa agaaaaagga gcccattgaa aagaaagtgg 181 tttcttctaa aaccaaaaaa gtgacaaaaa atgaggagcc ttctgaggaa gaaatagatg 241 ctcctaagcc caagaagatg aagaaagaaa aggaaatgaa tggagaaact agagagaaaa 301 gccccaaact gaagaatgga tttcctcatc ctgaaccgga ctgtaacccc agtgaagctg 361 ccagtgaaga aagtaacagt gagatagagc aggaaatacc tgtggaacaa aaagaaggcg 421 ctttctctaa ttttcccata tctgaagaaa ctattaaact tctcaaaggc cgaggagtga 481 ccttcctatt tcctatacaa gcaaagacat tccatcatgt ttacagcggg aaggacttaa 541 ttgcacaggc acggacagga actgggaaga cattctcctt tgccatccct ttgattgaga 601 aacttcatgg ggaactgcaa gacaggaaga gaggccgtgc ccctcaggta ctggttcttg 661 cacctacaag agagttggca aatcaagtaa gcaaagactt cagtgacatc acaaaaaagc 721 tgtcagtggc ttgtttttat ggtggaactc cctatggagg tcaatttgaa cgcatgagga 781 atgggattga tatcctggtt ggaacaccag gtcgtatcaa agaccacata cagaatggca 841 aactagatct caccaaactt aagcatgttg tcctggatga agtggaccag atgttggata 901 tgggatttgc tgatcaagtg gaagagattt taagtgtggc atacaagaaa gattctgaag 961 acaatcccca aacattgctt ttttctgcaa cttgccctca ttgggtattt aatgttgcca 1021 agaaatacat gaaatctaca tatgaacagg tggacctgat tggtaaaaag actcagaaaa 1081 cggcaataac tgtggagcat ctggctatta agtgccactg gactcagagg gcagcagtta 1141 ttggggatgt catccgagta tatagtggtc atcaaggacg cactatcatc ttttgtgaaa 1201 ccaagaaaga agcccaggag ctgtcccaga attcagctat aaagcaggat gctcagtcct 1261 tgcatggaga cattccacag aagcaaaggg aaatcaccct gaaaggtttt agaaatggta 1321 gttttggagt tttggtggca accaatgttg ctgcacgtgg gttagacatc cctgaggttg 1381 atttggttat acaaagctct ccaccaaagg atgtagagtc ctacattcat cgatccgggc 1441 ggacaggcag agctggaagg acgggggtgt gcatctgctt ttatcagcac aaggaagaat 1501 atcagttagt acaagtggag caaaaagcgg gaattaagtt caaacgaata ggtgttcctt 1561 ctgcaacaga aataataaaa gcttccagca aagatgccat caggcttttg gattccgtgc 1621 ctcccactgc cattagtcac ttcaaacaat cagctgagaa gctgatagag gagaagggag 1681 ctgtggaagc tctggcagca gcactggccc atatttcagg tgccacgtcc gtagaccagc 1741 gctccttgat caactcaaat gtgggttttg tgaccatgat cttgcagtgc tcaattgaaa 1801 tgccaaatat tagttatgct tggaaagaac ttaaagagca gctgggcgag gagattgatt 1861 ccaaagtgaa gggaatggtt tttctcaaag gaaagctggg tgtttgcttT GATGTACCTA 1921 CCGCATCAGT AACAGAAATA CAGGAGAAAT GGCATGATTC ACGACGCTGG CAGCTCTCTG 1981 TGGCCACAGA GCAACCAGAA CTGGAAGGAC CACGGGAAGG ATATGGAGGC TTCAGGGGAC 2041 AGCGGGAAGG CAGTCGAGGC TTCAGGGGAC AGCGGGACGG AAACAGAAGA TTCAGAGGAC 2101 AGCGGGAAGG CAGTAGAGGC CCGAGAGGAC AGCGATCAGG AGGTGGCAAC AAAAGTAACA 2161 GATCCCAAAA CAAAGGCCAG AAGCGGAGTT TCAGTAAAGC ATTTGGTCAA TACCCAACTT 2221 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2281 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2341 CTTGTGGAAA GGACGAGCCG AGAACACCGG AACCAAATAT ACGCGTTAAG TCgacaatca 2401 acctctggat tacaaaattt gtgaaagatt