Construct: ORF TRCN0000481522
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013051.1_s317c1
- Derived from:
- ccsbBroadEn_11885
- DNA Barcode:
- TTGACAGGACTAGAGCATCGAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGLV2-14 (28815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481522
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 49.7% | 45.7% | (many diffs) |
| 2 | human | 723778 | MIR650 | microRNA 650 | NR_030755.1 | 6.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgggctctg ctattcctca ccctcctcac tcagggcaca gggtcctggg 121 cccagtctgc cctgactcag cctgcctccg tgtctgggtc tcctggacag tcgatcacca 181 tctcctgcac tggaaccagc agtgacgttg gtggttataa ctatgtctcc tggtaccaac 241 agcacccagg caaagccccc aaactcatga tttatgatgt cagtaatcgg ccctcagggg 301 tttctaatcg cttctctggc tccaagtctg gcaacacggc ctccctgacc atctctgggc 361 TCCAGGCTGA GGACGAGGCT GATTATTACT GCAGCTCATA TACAAGCAGC ACCACTCCTG 421 TGGTATTCGG CGGAGGGACC AAGCTGACCG TCCTAGGTCA GCCCAAGGCT GCCCCCTCGG 481 TCACTCTGTT CCCGCCCTCC TCTGAGGAGC TTCAAGCCAA CAAGGCCACA CTGGTAAGTG 541 ACTTCTACCC GGGAGCCGTG ACAGTGGCCT GGAAGGCAGA TAGCAGCCCC GTCAAGGCGG 601 GAGTGGAGAC CACCACACCC TCCAAACAAA GCAACAACAA GTACGCGGCC AGCAGCTACC 661 TGAGCCTGAC GCCTGAGCAG TGGAAGTCCC ACAGAAGCTA CAGCTGCCAG GTCACGCATG 721 AAGGGAGCAC CGTGGAGAAG ACAGTGGCCC CTACAGAATG TTCATACCCA ACTTTCTTGT 781 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 841 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 901 GAAAGGACGA TTGACAGGAC TAGAGCATCG AGGTACGCGT TAAGTCgaca atcaacctct 961 ggattacaaa atttgtgaaa gatt