Construct: ORF TRCN0000481523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011381.1_s317c1
- Derived from:
- ccsbBroadEn_03377
- DNA Barcode:
- CCCATACCTCCCCAGTGTTGTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD244 (51744)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481523
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51744 | CD244 | CD244 molecule | NM_016382.4 | 100% | 100% | |
| 2 | human | 51744 | CD244 | CD244 molecule | NM_001166663.2 | 98.6% | 98.6% | 379_393del |
| 3 | human | 51744 | CD244 | CD244 molecule | XM_011509622.2 | 88.7% | 88.4% | (many diffs) |
| 4 | human | 51744 | CD244 | CD244 molecule | XM_011509621.2 | 87.6% | 87.2% | (many diffs) |
| 5 | human | 51744 | CD244 | CD244 molecule | NM_001166664.2 | 74.7% | 74.7% | 378_379ins276 |
| 6 | human | 51744 | CD244 | CD244 molecule | XM_011509623.3 | 44.4% | 41.1% | (many diffs) |
| 7 | human | 51744 | CD244 | CD244 molecule | XR_001737229.1 | 39.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1161
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggggcaagtg gtcaccctca tactcctcct gctcctcaag gtgtatcagg 121 gcaaaggatg ccagggatca gctgaccatg tggttagcat ctcgggagtg cctcttcagt 181 tacaaccaaa cagcatacag acgaaggttg acagcattgc atggaagaag ttgctgccct 241 cacaaaatgg atttcatcac atattgaagt gggagaatgg ctctttgcct tccaatactt 301 ccaatgatag attcagtttt atagtcaaga acttgagtct tctcatcaag gcagctcagc 361 agcaggacag tggcctctac tgcctggagg tcaccagtat atctggaaaa gttcagacag 421 ccacgttcca ggtttttgta tttgataaag ttgagaaacc ccgcctacag gggcagggga 481 agatcctgga cagagggaga tgccaagtgg ctctgtcttg cttggtctcc agggatggca 541 atgtgtccta tgcttggtac agagggagca agctgatcca gacagcaggg aacctcacct 601 acctggacga ggaggttgac attaatggca ctcacacata tacctgcaat gtcagcaatc 661 ctgttagctg ggaaagccac accctgaatc tcactcagga ctgtcagaat gcccatcagg 721 aattcagatt ttggccgttt ttggtgatca tcgtgattct aagcgcactg ttccttggCA 781 CCCTTGCCTG CTTCTGTGTG TGGAGGAGAA AGAGGAAGGA GAAGCAGTCA GAGACCAGTC 841 CCAAGGAATT TTTGACAATT TACGAAGATG TCAAGGATCT GAAAACCAGG AGAAATCACG 901 AGCAGGAGCA GACTTTTCCT GGAGGGGGGA GCACCATCTA CTCTATGATC CAGTCCCAGT 961 CTTCTGCTCC CACGTCACAA GAACCTGCAT ATACATTATA TTCATTAATT CAGCCTTCCA 1021 GGAAGTCTGG ATCCAGGAAG AGGAACCACA GCCCTTCCTT CAATAGCACT ATCTATGAAG 1081 TGATTGGAAA GAGTCAACCT AAAGCCCAGA ACCCTGCTCG ATTGAGCCGC AAAGAGCTGG 1141 AGAACTTTGA TGTTTATTCC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1201 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1261 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCCA TACCTCCCCA 1321 GTGTTGTTCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt