Construct: ORF TRCN0000481536
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013523.1_s317c1
- Derived from:
- ccsbBroadEn_09317
- DNA Barcode:
- CCACCCTGATCGGTACCGTTCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RSAD2 (91543)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481536
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91543 | RSAD2 | radical S-adenosyl methioni... | NM_080657.5 | 99.9% | 99.7% | 125T>G |
| 2 | human | 91543 | RSAD2 | radical S-adenosyl methioni... | XM_011510415.2 | 77.2% | 65% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1149
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggtgcttaca cctgctgctt ttgctgggaa gctcttgagt gtgttcaggc 121 aacctctgag ctctctgtgg aggagcctgg tcccgctgtt ctgctggctg agggcaacct 181 tctggctgcg agctaccaag aggagaaagc agcagctggt cctgagaggg ccagatgaga 241 ccaaagagga ggaagaggac cctcctctgc ccaccacccc aaccagcgtc aactatcact 301 tcactcgcca gtgcaactac aaatgcggct tctgtttcca cacagccaaa acatcctttg 361 tgctgcccct tgaggaagca aagagaggat tgcttttgct taaggaagct ggtatggaga 421 agatcaactt ttcaggtgga gagccatttc ttcaagaccg gggagaatac ctgggcaagt 481 tggtgaggtt ctgcaaagta gagttgcggc tgcccagcgt gagcatcgtg agcaatggaa 541 gcctgatccg ggagaggtgg ttccagaatt atggtgagta tttggacatt ctcgctatct 601 cctgtgacag ctttgacgag gaagtcaatg tccttattgg ccgtggccaa ggaaagaaga 661 accatgtgga aaaccttcaa aagctgagga ggtggtgtag ggattataga gtcgctttca 721 agataaattc tgtcaTTAAT CGTTTCAACG TGGAAGAGGA CATGACGGAA CAGATCAAAG 781 CACTAAACCC TGTCCGCTGG AAAGTGTTCC AGTGCCTCTT AATTGAGGGT GAGAATTGTG 841 GAGAAGATGC TCTAAGAGAA GCAGAAAGAT TTGTTATTGG TGATGAAGAA TTTGAAAGAT 901 TCTTGGAGCG CCACAAAGAA GTGTCCTGCT TGGTGCCTGA ATCTAACCAG AAGATGAAAG 961 ACTCCTACCT TATTCTGGAT GAATATATGC GCTTTCTGAA CTGTAGAAAG GGACGGAAGG 1021 ACCCTTCCAA GTCCATCCTG GATGTTGGTG TAGAAGAAGC TATAAAATTC AGTGGATTTG 1081 ATGAAAAGAT GTTTCTGAAG CGAGGAGGAA AATACATATG GAGTAAGGCT GATCTGAAGC 1141 TGGATTGGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCACCC TGATCGGTAC CGTTCCGGAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt