Construct: ORF TRCN0000481541

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006082.1_s317c1
Derived from:
ccsbBroadEn_07874
DNA Barcode:
CCTGCTTCGCTACTTTACTCATCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMG5 (23381)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481541
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23381 SMG5 SMG5 nonsense mediated mRNA... NM_015327.3 99.9% 99.8% 8A>C;3010A>G
2 human 23381 SMG5 SMG5 nonsense mediated mRNA... NM_001323615.1 95.4% 95.2% 8A>C;1114_1115ins138;2872A>G
3 human 23381 SMG5 SMG5 nonsense mediated mRNA... NM_001323614.1 94.7% 94.5% 8A>C;2282_2283ins159;2851A>G
4 human 23381 SMG5 SMG5 nonsense mediated mRNA... NM_001323616.1 93.4% 93.4% 0_1ins198;2812A>G
5 human 23381 SMG5 SMG5 nonsense mediated mRNA... NM_001323617.1 93.4% 93.4% 0_1ins198;2812A>G
6 mouse 229512 Smg5 Smg-5 homolog, nonsense med... NM_178246.3 88.4% 93% (many diffs)
7 mouse 229512 Smg5 Smg-5 homolog, nonsense med... XM_006501343.3 87% 91.6% (many diffs)
8 mouse 229512 Smg5 Smg-5 homolog, nonsense med... XM_011240087.2 82.6% 86.9% (many diffs)
9 mouse 229512 Smg5 Smg-5 homolog, nonsense med... XM_006501345.2 65.1% 67.9% (many diffs)
10 mouse 229512 Smg5 Smg-5 homolog, nonsense med... XM_006501346.3 56.9% 56.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3114
ORF length:
3048
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag cccaggcccc cccacagggg agagcagcga gcccgaagca aaagtcctcc
121acactaagcg gctttaccgg gctgtggtgg aggctgtgca tcgacttgac ctcatccttt
181gcaacaaaac tgcttatcaa gaagtattca aaccagaaaa cattagcctg aggaacaagc
241tgcgtgagct ctgcgtcaag cttatgttcc tgcacccagt ggactatggg agaaaggctg
301aggagctgct gtggagaaag gtatactatg aagttatcca gcttatcaag actaacaaaa
361agcacatcca cagccggagc actttggaat gtgcctacag gacgcacctg gttgctggta
421ttggcttcta ccagcatctc cttctctata tccagtccca ctaccagctg gaactgcagt
481gctgcatcga ctggacccat gtcactgacc ccctcatagg atgcaagaag ccagtgtctg
541cctcagggaa ggagatggat tgggcacaga tggcatgtca ccgatgtctg gtgtatctgg
601gggatttgtc ccgatatcag aatgaattag ctggcgtaga taccgagctg ctagccgaga
661gattttacta ccaagccctg tcagtagctc ctcagattgg aatgcccttc aatcagctgg
721gcaccctggc aggcagcaag tactataatg tggaagccat gtattgctac ctgcgctgca
781tccagtcaga agtgtccttt gagggagcct atgggaacct caagcggctg tatgacaagg
841cagccaaaat gtaccaccaa ctgaagaagt gtgagactcg gaaactgtct cctggcaaaa
901agcgatgtaa agacattaaa aggttgctag tgaactttat gtatctgcaa agcctcctac
961agcccaaaag cagctccgtg gactcagagc tgacctcact ttgccagtca gtcctggagg
1021acttcaacct ctgcctcttc tacctgccct cctcacccaa cctcagcctg gccagtgagg
1081atgaggagga gtatgagagt ggatatgctt tcctcccgga ccttctcatc tttcaaatgg
1141tcatcatctg ccttatgtgt gtgcacagct tggagagagc aggatccaag cagtacagtg
1201cagccattgc cttcaccctg gccctctttt cccacctcgt caatcatgtc aacatacggc
1261tgcaggctga gctggaagag ggcgagaatc ccgtcccggc attccagagt gatggcacag
1321atgaaccaga gtccaaggaa cctgtggaga aagaggagga gccagatcct gagcctcctc
1381ctgtaacacc ccaagtgggt gagggcagaa agagccgtaa gttctctcgc ctctcctgtc
1441tccgccgtcg ccgccaccca cccaaagttg gtgatgacag tgacctgagt gaaggctttg
1501aatcggactc aagccatgac tcagcccggg ccagtgaggg ctcagacagt ggctctgaca
1561agagtcttga aggtggggga acggcctttg atgctgaaac agactcggaa atgaatagcc
1621aggagtcccg atcagacttg gaagatatgg aggaagagga ggggacacgg tcaccaaccc
1681tggagccccc tcggggcaga tcagaggctc ccgattccct caatggccca ctgggcccca
1741gtgaggctag cattgccagc aatctacaag ccatgtccac ccagatgttc cagactaagc
1801gctgcttccg actggccccc acctttagca acctgctcct ccagcccacc accaaccctc
1861atacctcggc cagccacagg ccttgcgtca atggggatgt agacaagcct tcagagccag
1921cctctgagga gggctctgag tcggagggga gtgagtccag tggacgctcc tgtcggaatg
1981agcgcagcat ccaggagaag cttcaggtcc tgatggccga aggtctgctt cctgctgtga
2041aagtcttcct ggactggctt cggaccaacc ccgacctcat catcgtgtgt gcgcagagct
2101ctcaaagtct gtggaaccgc ctgtctgtgt tgctgaatct gttgcctgct gctggtgaac
2161tccaggagtc tggcctggcc ttgtgtcctg aggtccaaga tcttcttgaa ggttgtgaac
2221tgcctgacct cccctctagc cttctgctcc cagaggacat ggctcttcgt aacctgcccc
2281cgctccgagc tgcccacaga cgctttaact ttgacacgga tcggcccctg ctcagcacct
2341tagaggagtc agtggtgcgc atctgctgca tccgcagctt tggtcatttc atcgcccgcc
2401tgcaaggcag catcctgcag ttcaacccag aggttggcat cttcgtcagc attgcccagt
2461ctgagcagga gagcctgctg cagcaggccc aggcacagtt ccgaatggca caggaggaag
2521ctcgtcggaa caggctcatg agagacatgg ctcagctacg acttcagctc gaagtgtctc
2581agctggaggg cagcctgcag cagcccaagg cccagtcagc catgtctccc tacctcgtcc
2641ctgacaccca ggccctctgc caccatctcc ctgtcatccg ccaactggcc accagtggcc
2701gcttcattgT CATCATCCCA AGGACAGTGA TCGATGGCCT GGATTTGCTG AAGAAGGAAC
2761ACCCAGGGGC CCGGGATGGG ATTCGGTACC TGGAGGCAGA GTTTAAAAAA GGAAACAGGT
2821ACATTCGCTG CCAGAAAGAG GTGGGAAAGA GCTTTGAGCG GCATAAGCTG AAGAGGCAGG
2881ATGCAGATGC CTGGACTCTC TATAAGATCC TAGACAGCTG CAAACAGCTG ACTCTGGCCC
2941AGGGGGCAGG TGAGGAGGAT CCGAGTGGCA TGGTGACCAT CATCACAGGC CTTCCACTGG
3001ACAACCCCAG CGTGCTTTCA GGCCCCATGC AGGCAGCCCT GCAGGCCGCT GCCCACGCCA
3061GTGTGGACAT CAAGGATGTT CTGGACTTCT ACAAGCAGTG GAAGGAAATT GGTTGCCCAA
3121CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
3181ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
3241TATCTTGTGG AAAGGACGAC CTGCTTCGCT ACTTTACTCA TCAACGCGTT AAGTCgacaa
3301tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)