Construct: ORF TRCN0000481555
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018264.1_s317c1
- Derived from:
- ccsbBroadEn_10806
- DNA Barcode:
- AGGCGAGCCAACTGCTTGACAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERBB3 (2065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481555
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2065 | ERBB3 | erb-b2 receptor tyrosine ki... | NM_001005915.1 | 52.3% | 44.4% | (many diffs) |
| 2 | human | 2065 | ERBB3 | erb-b2 receptor tyrosine ki... | NM_001982.3 | 24.6% | 24.5% | 992G>T;994_4026del |
| 3 | mouse | 13867 | Erbb3 | erb-b2 receptor tyrosine ki... | NM_010153.1 | 21.6% | 22.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggcgaacgac gctctgcagg tgctgggctt gcttttcagc ctggcccggg 121 gctccgaggt gggcaactct caggcagtgt gtcctgggac tctgaatggc ctgagtgtga 181 ccggcgatgc tgagaaccaa taccagacac tgtacaagct ctacgagagg tgtgaggtgg 241 tgatggggaa ccttgagatt gtgctcacgg gacacaatgc cgacctctcc ttcctgcagt 301 ggattcgaga agtgacaggc tatgtcctcg tggccatgaa tgaattctct actctaccat 361 tgcccaacct ccgcgtggtg cgagggaccc aggtctacga tgggaagttt gccatcttcg 421 tcatgttgaa ctataacacc aactccagcc acgctctgcg ccagctccgc ttgactcagc 481 tcaccgagat tctgtcaggg ggtgtttata ttgagaagaa cgataAGCTT TGTCACATGG 541 ACACAATTGA CTGGAGGGAC ATCGTGAGGG ACCGAGATGC TGAGATAGTG GTGAAGGACA 601 ATGGCAGAAG CTGTCCCCCC TGTCATGAGG TTTGCAAGGG GCGATGCTGG GGTCCTGGAT 661 CAGAAGACTG CCAGACATTG ACCAAGACCA TCTGTGCTCC TCAGTGTAAT GGTCACTGCT 721 TTGGGCCCAA CCCCAACCAG TGCTGCCATG ATGAGTGTGC CGGGGGCTGC TCAGGCCCTC 781 AGGACACAGA CTGCTTTGCC TGCCGGCACT TCAATGACAG TGGAGCCTGT GTACCTCGCT 841 GTCCACAGCC TCTTGTCTAC AACAAGCTAA CTTTCCAGCT GGAACCCAAT CCCCACACCA 901 AGTATCAGTA TGGAGGAGTT TGTGTAGCCA GCTGTCCCCA TAACTTTGTG GTGGATCAAA 961 CATCCTGTGT CAGGGCCTGT CCTCCTGACA AGATGGAAGT AGATAAAAAT GGGCTCAAGA 1021 TGTGTGAGCC TTGTGGGGGA CTATGTCCCA AAGCCTTTTA CCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGAAGGCGA GCCAACTGCT TGACAGGTAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt