Construct: ORF TRCN0000481568
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013616.2_s317c1
- Derived from:
- ccsbBroadEn_08394
- DNA Barcode:
- TCCAAAGTCAAATCTCAACTGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UGT1A6 (54578)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481568
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54578 | UGT1A6 | UDP glucuronosyltransferase... | NM_001072.4 | 99.9% | 99.8% | 962T>C |
| 2 | human | 54578 | UGT1A6 | UDP glucuronosyltransferase... | NM_205862.2 | 49.7% | 49.6% | 0_1ins801;161T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgcctcctt cgctcatttc agagaatttc tgcaggggtt ttcttcttag 121 cactttgggg catggttgta ggtgacaagc tgctggtggt ccctcaggac ggaagccact 181 ggcttagtat gaaggatata gttgaggttc tcagtgaccg gggtcatgag attgtagtgg 241 tggtgcctga agttaatttg cttttgaaag aatccaaata ctacacaaga aaaatctatc 301 cagtgccgta tgaccaagaa gagctgaaga accgttacca atcatttgga aacaatcact 361 ttgctgagcg atcattccta actgctcctc agacagagta caggaataac atgattgtta 421 ttggcctgta cttcatcaac tgccagagcc tcctgcagga cagggacacc ctgaacttct 481 ttaaggagag caagtttgat gctcttttca cagacccagc cttaccctgt ggggtgatcc 541 tggctgagta tttgggccta ccatctgtgt acctcttcag gggttttccg tgttccctgg 601 agcatacatt cagcagaagc ccagaccctg tgtcctacat tcccaggtgc tacacaaagt 661 tttcagacca catgactttt tcccaacgag tggccaactt ccttgttaat ttgttggagc 721 cctatctatt ttattgtctg ttttcaaagt atgaagaact cgcatcagct gtcctcaaga 781 gagatgtgga tataatcacc ttatatcaga aggtctctgt ttggctgtta agatatgact 841 ttgtgcttga atatcctagg ccggtcatgc ccaacatggt cttcattgga ggtatcaact 901 gtaagaagag gaaagacttg tctcaggaat ttgaagccta cattaatgct tctggagaac 961 atggaattgt ggttttctct ttgggatcaa tggtctcaga aattccagag aagaaagcta 1021 tggcaactgc tgatgctttg ggcaaaatcc ctcagacagt cctgtggcgg tacactggaa 1081 cccgaccatc gaatcttgcg aacaacacga tacttgttaa gtggctaccc caaaacgatc 1141 tgcttggtca cccgatgacc cgtgccttta TCACCCATGC TGGTTCCCAT GGTGTTTATG 1201 AAAGCATATG CAATGGCGTT CCCATGGTGA TGATGCCCTT GTTTGGTGAT CAGATGGACA 1261 ATGCAAAGCG CATGGAGACT AAGGGAGCTG GAGTGACCCT GAATGTTCTG GAAATGACTT 1321 CTGAAGATTT AGAAAATGCT CTAAAAGCAG TCATCAATGA CAAAAGTTAC AAGGAGAACA 1381 TCATGCGCCT CTCCAGCCTT CACAAGGACC GCCCGGTGGA GCCGCTGGAC CTGGCCGTGT 1441 TCTGGGTGGA GTTTGTGATG AGGCACAAGG GCGCGCCACA CCTGCGCCCC GCAGCCCACG 1501 ACCTCACCTG GTACCAGTAC CATTCCTTGG ACGTGATTGG TTTCCTCTTG GCCGTCGTGC 1561 TGACAGTGGC CTTCATCACC TTTAAATGTT GTGCTTATGG CTACCGGAAA TGCTTGGGGA 1621 AAAAAGGGCG AGTTAAGAAA GCCCACAAAT CCAAGACCCA TTGCCCAACT TTCTTGTACA 1681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1741 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1801 AGGACGATCC AAAGTCAAAT CTCAACTGCA AACGCGTTAA GTCgacaatc aacctctgga 1861 ttacaaaatt tgtgaaagat t