Construct: ORF TRCN0000481573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009939.1_s317c1
- Derived from:
- ccsbBroadEn_13027
- DNA Barcode:
- CCTTATCCTGTAGTCACAAGGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM83F (113828)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481573
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113828 | FAM83F | family with sequence simila... | NM_138435.4 | 66.3% | 66.2% | 1_504del;1045C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1062
- ORF length:
- 996
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cctcttcact gatggtgata tctttcaaga cattgtggat gctgcctgta 121 agcgccgggt cccagtgtac atcatcctgg acgaggcagg agtgaagtat ttcctggaga 181 tgtgtcagga cctgcagctc actgacttcc ggattcggaa catccgtgtc cgctctgtga 241 caggcgtcgg cttctacatg cccatgggga ggatcaaggg gaccctgtca tcaaggttcc 301 tgatggtgga cggtgacaaa gtggccactg gatcttacag gttcacctgg agttcctccc 361 atgtggacag aaacctcctc ctgctcctga caggacagaa cgtagagccc tttgacacgg 421 agttccggga gctgtacgcc atctccgagg aggtggactt gtaccggcag ctgagcctgg 481 cgggcagggt tggcctccat tactcctcca ctgtggctcg aaagcttatc aaccccaagt 541 acgccttggt gtcaggctgc cgccacccgc ctGGGGAGAT GATGCGCTGG GCTGCCCGGC 601 AACAGTGGGA GGCGGGCGGC AACCCGGAGG GGCAGGAGGA GGGCGCCAGC GGTGGCGAGT 661 CGGCCTGGCG CCTGGAGAGC TTCCTGAAAG ACCTGGTTAC GGTGGAGCAG GTGCTGCCCC 721 CCGTGGAGCC CATCCCCTTG GGAGAGCTGA GCCAGAAGGA TGGCAGGATG GTCTCTCACA 781 TGCACAGAGA CCTGAAGCCC AAATCCCGAG AGGCACCCAG CCGAAACGGC ATGGGAGAAG 841 CGGCCCGGGG GGAGGCCGCC CCCGCCAGGC GCTTCAGCAG CAGGCTCTTC AGTCGCCGAG 901 CCAAGAGGCC TGCGGCGCCC AATGGCATGG CCAGCTCTGT CTCCACCGAG ACCTCTGAAG 961 TGGAGTTTCT GACGGGGAAG AGGCCCAACG AGAATTCCAG TGCTGACATC TCAGGTAAAA 1021 CAAGTCCCAG TTCTGCCAAG CCTAGCAACT GTGTGATTTC CTGCCCAACT TTCTTGTACA 1081 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1141 AATGAACTAG CCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1201 AGGACGACCT TATCCTGTAG TCACAAGGGG TACGCGTTAA GTCgacaatc aacctctgga 1261 ttacaaaatt tgtgaaagat t