Construct: ORF TRCN0000481579
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001311.2_s317c1
- Derived from:
- ccsbBroadEn_12564
- DNA Barcode:
- ACCGAGGAACCCACGTTACATCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOX5 (79400)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481579
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79400 | NOX5 | NADPH oxidase 5 | NM_001184780.2 | 98.3% | 98.3% | (many diffs) |
| 2 | human | 79400 | NOX5 | NADPH oxidase 5 | NM_001184779.2 | 97.4% | 97.4% | (many diffs) |
| 3 | human | 79400 | NOX5 | NADPH oxidase 5 | NM_024505.4 | 93.8% | 93.8% | (many diffs) |
| 4 | human | 79400 | NOX5 | NADPH oxidase 5 | NR_033672.2 | 25.2% | (many diffs) | |
| 5 | human | 79400 | NOX5 | NADPH oxidase 5 | NR_033671.3 | 25% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2226
- ORF length:
- 2157
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgccgag gaggatgcca ggtggctccg gtgggtgact cagcagttta 121 agaccattgc aggagaagat ggggagatca gcctgcaaga attcaaagca gctctgcatg 181 tgaaagagtc cttctttgca gagcgattct ttgccctatt tgactccgat agaagtggca 241 ccatcaccct ccaggagctg caggaggcac tgaccctgct catccatggc agccccatgg 301 acaaactcaa attcctcttc caggtgtatg acatcgatgg cagtggctcc attgacccgg 361 atgagctgcg cactgtgctg cagtcgtgtc tgcgcgagag cgccatctcg ctgcctgacg 421 agaagctgga ccagctgacg ctggcgctct tcgaatcggc cgacgcggac ggcaacgggg 481 ccatcacctt cgaggagctc cgggacgagc tgcagcgctt ccccggagtc atggagaacc 541 tgaccatcag cgctgcccac tggctgacgg cccccgcccc ccgcccacgc ccgcgccggc 601 cgcgccagct gacccgcgcc tactggcaca accaccgcag ccagctgttc tgcctggcca 661 cctatgcagg cctccacgtg ctgctcttcg ggctggcggc cagcgcgcac cgggacctcg 721 gcgccagcgt catggtggcc aagggctgtg gccagtgcct caacttcgac tgcagcttca 781 tcgcggtgct gatgctcaga cgctgcctca cctggctgcg ggccacgtgg ctggctcaag 841 tcctaccact ggaccagaac atccagttcc accagcttat gggctacgtg gtagtggggc 901 tgtccctcgt gcacactgtg gctcacactg tgaactttgt actccaggct caggcggagg 961 ccagcccttt ccagttctgg gagctgctgc tcaccacgag gcctggcatt ggctgggtac 1021 acggttcggc ctccccgaca ggtgtcgctc tgctgctgct gctcctcttc atgttcatct 1081 gctccagttc ctgcatccgc aggagtggcc actttgaggt gttctattgg actcacctgt 1141 cctacctcct cgtgtggctt ctgctcatct ttcatgggcc caacttctgg aagtggctgc 1201 tggtgcctgg aatcttgttt ttcctggaga aggccatcgg actggcagtg tcccgcatgg 1261 cagccgtgtg catcatggaa gtcaacctcc tcccctccaa ggtcactcat ctcctcatca 1321 agcggccccc tttttttcac tatagacctg gtgactactt gtatctgaac atccccacca 1381 ttgctcgcta tgagtggcac cccttcacca tcagcagtgc tcctgagcag aaagacacta 1441 tctggctgca cattcggtcc caaggccagt ggacaaacag gctgtatgag tccttcaagg 1501 catcagaccc actgggccgt ggttctaaga ggctgtcgag gagtgtgaca atgagaaaga 1561 gtcaaaggtc gtccaagggc tctgagatac ttttggagaa acacaaattc tgtaacatca 1621 agtgctacat cgatgggcct tatgggaccc ccacccgcag gatctttgcc tctgagcatg 1681 ccgtgctcat cggggcaggc atcggcatca ccccctttgc ttccattctg cagagtatca 1741 tGTACAGGCA CCAGAAAAGA AAGCATACTT GCCCCAGCTG CCAGCACTCC TGGATCGAAG 1801 GTGTCCAAGA CAACATGAAG CTCCATAAGG TGGACTTTAT CTGGATCAAC AGAGACCAGC 1861 GGTCTTTCGA GTGGTTTGTG AGCCTGCTGA CTAAACTGGA GATGGACCAG GCCGAGGAGG 1921 CTCAATACGG CCGCTTCCTG GAGCTGCATA TGTACATGAC ATCTGCACTG GGCAAGAATG 1981 ACATGAAGGC CATTGGCCTG CAGATGGCCC TTGACCTCCT GGCCAACAAG GAGAAGAAAG 2041 ACTCCATCAC GGGGCTGCAG ACGCGCACCC AGCCTGGGCG GCCTGACTGG AGCAAGGTGT 2101 TCCAGAAAGT GGCTGCTGAG AAGAAGGGCA AGGTGCAGGT CTTCTTCTGT GGCTCCCCAG 2161 CTCTGGCCAA GGTGCTGAAG GGCCATTGTG AGAAGTTCGG CTTCAGATTT TTCCAAGAGA 2221 ATTTCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2281 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2341 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACCGAGGAA CCCACGTTAC ATCCGACGCG 2401 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt