Construct: ORF TRCN0000481591
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007449.2_s317c1
- Derived from:
- ccsbBroadEn_04700
- DNA Barcode:
- ATTTTTTGCGGAGTGCCATACCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TM4SF19 (116211)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481591
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116211 | TM4SF19 | transmembrane 4 L six famil... | NM_138461.4 | 100% | 100% | |
| 2 | human | 116211 | TM4SF19 | transmembrane 4 L six famil... | NM_001204898.2 | 87.5% | 87.5% | 201_202ins78 |
| 3 | human | 116211 | TM4SF19 | transmembrane 4 L six famil... | NM_001204897.2 | 85.3% | 58.8% | 445_446insGTAG;624_726del |
| 4 | human | 100534611 | TM4SF19-TCTEX1D2 | TM4SF19-TCTEX1D2 readthroug... | NR_037950.1 | 61.3% | 1_158del;786_1022del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 696
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgtcctct ccctgcacgc aggcaagctc acggacttgc tcccgtatcc 121 tgggactgag ccttgggact gcagccctgt ttgctgctgg ggccaacgtg gcactcctcc 181 ttcctaactg ggatgtcacc tacctgttga ggggcctcct tggcaggcat gccatgctgg 241 gaactgggct ctggggagga ggcctcatgg tactcactgc agctaTCCTC ATCTCCTTGA 301 TGGGCTGGAG ATACGGCTGC TTCAGTAAGA GTGGGCTCTG TCGAAGCGTG CTTACTGCTC 361 TGTTGTCAGG TGGCCTGGCT TTACTTGGAG CCCTGATTTG CTTTGTCACT TCTGGAGTTG 421 CTCTGAAAGA TGGTCCTTTT TGCATGTTTG ATGTTTCATC CTTCAATCAG ACACAAGCTT 481 GGAAATATGG TTACCCATTC AAAGACCTGC ATAGTAGGAA TTATCTGTAT GACCGTTCGC 541 TCTGGAACTC CGTCTGCCTG GAGCCCTCTG CAGCTGTTGT CTGGCACGTG TCCCTCTTCT 601 CCGCCCTTCT GTGCATCAGC CTGCTCCAGC TTCTCCTGGT GGTCGTTCAT GTCATCAACA 661 GCCTCCTGGG CCTTTTCTGC AGCCTCTGCG AGAAGTTGCC AACTTTCTTG TACAAAGTGG 721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 841 AATTTTTTGC GGAGTGCCAT ACCGTACGCG TTAAGTCgac aatcaacctc tggattacaa 901 aatttgtgaa agatt