Construct: ORF TRCN0000481613
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014067.1_s317c1
- Derived from:
- ccsbBroadEn_03702
- DNA Barcode:
- TGCTGATTCGTTCTCTACGATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGA7 (56108)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481613
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56108 | PCDHGA7 | protocadherin gamma subfami... | NM_032087.3 | 100% | 100% | |
| 2 | human | 56108 | PCDHGA7 | protocadherin gamma subfami... | NM_018920.4 | 87.2% | 86.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2520
- ORF length:
- 2451
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tgggcaccat ggcggctcag ccgaggggcg gggactacag aggattcttc ctgctctcca 121 tcctcctggg gaccccctgg gaagcctggg caggacgtat tctctactcc gtgtcggagg 181 agacggacaa agggtccttt gtgggagaca tcgccaagga cctggggctg gagccccggg 241 agctggcgga gcgcggagtc cgcatcatct ccagaggtag gacgcagctt ttcgccctga 301 accagcgcag cggcagcttg gtcactgcgg gcaggataga ccgggaagag atctgcgctc 361 agagtgcgcg gtgtctggta aactttaaca tcctgatgga agataaaatg aatctttacc 421 ctatagacgt ggaaataata gatattaatg acaacgttcc aagattcttg acggaagaaa 481 taaatgtaaa aataatggag aatacagctc ctggggttcg gtttccgtta agcgaggctg 541 gggatccaga tgtgggcacg aactccctcc agagttacca gctcagcccc aatcgccact 601 tctccctggc tgtgcaaagt ggagacgatg aaactaagta cccggaactg gtgctggagc 661 gggtgctgga ccgggaggaa gagcgggttc accacctggt cctcacagcc tctgatggcg 721 gcgacccgcc ccgatccagc accgcccaca tccaggtgac agtggtggat gtgaatgacc 781 acacgcctgt cttctctctg cctcagtacc aagtaactgt ccccgagaat gtgccagtgg 841 gtacaagact gctcacggta catgctatcg acctggacga gggagtcaat ggggaagtga 901 catattcttt tcggaaaata actcctaaac ttccaaagat gtttcatctg aactcgctta 961 caggagaaat atcaacttta gaaggattag attatgaaga aactgccttc tatgaaatgg 1021 aggttcaggc tcaagatggt cctggtagtc tgacaaaggc aaaagtactg atcacagttt 1081 tagatgtaaa tgataatgct ccagaagtga ctatgacgtc tttaagtagc tcaatccctg 1141 aagacacacc tcttgggaca gtcattgctc ttttctacct acaagacaga gattctggaa 1201 agaatggtga ggtgacctgc accattccag aaaacctacc ttttaaatta gaaaaatcaa 1261 tagataatta ttatagattg gtcacaacca aaaacttgga ccgggaaaca ctctctttgt 1321 ataacatcac actgaaagcc acagatggtg gaactcctcc cttgtccagg gaaactcaca 1381 tattcatgca ggtggcagac accaacgata acccacccac cttcccccac tcatcctact 1441 cagtctacat cgctgagaac aaccccagag gggcctccat tttcttagtg actgcacagg 1501 accacgacag tgaggataat gcccagatca cttattcctt ggccgaagac accatccagg 1561 gggctccagt gtcctcctat gtctccataa actctgacac tggagtcctg tacgcgctgc 1621 aatcctttga ttatgagcag ttgagagaac tacaactaag agtgactgca catgacagcg 1681 gggacccgcc tctcagcagc aacatgtcac tgagcctgtt cgtgctggac cagaatgaca 1741 acccgcccga gatcctgtac ccggccctcc ccacagatgg ttctactggc atggagctgg 1801 caccccgctc cgcagagccc ggctacctgg tgaccaaggt ggtggcggtg gacaaagatt 1861 caggccagaa cgcctggctg tcatacctcc tgcttaaggc cagcgagcca ggactctttg 1921 cggttgggct gtacacgggc gaggtgcgca cggctcgggc cctgctggac agagatgccc 1981 tcaagcagag cctcgtggtg gccgtccagg accacggtca gcctcctctg tcagccaccg 2041 tcacactcac cgtggctgtg gctgacagca TCCCCGAAGT CTTGGCCGAC CTGGGCAGCC 2101 TTGAGCCCTC CGACGGTCCT TACAACTATG ACCTCACGTT GTACCTGGTG GTGGCGGTGG 2161 CCACAGTCTC CTGCGTCTTC CTAGCCTTCG TCCTCGTACT GCTGGCGCTC AGGCTGCGGC 2221 GCTGGCACAA GTCACGCCTG CTGCAGGCTT CAGAAGGTGG CTTGGCGAAC GTGCCCACCT 2281 CGCACTTTGT GGGCATGGAC GGGGTGCAGG CTTTCCTGCA GACCTATTCC CATGAGGTCT 2341 CCCTCACCGC GGACTCTCGG AAGAGTCACC TGATCTTCCC CCAGCCCAAC TATGTAGACA 2401 TGCTCATCAG CCAGGAGAGC TGTGAGAAAA ATGATTCTTT GCTAACATCC GTAGATTTTC 2461 AGGAATGTAA AGAAAACCTG CCAAGTATTC AGGTGAGCCC AGCCCTTCCT TTATTTCCAT 2521 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2581 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2641 TTTATATATC TTGTGGAAAG GACGATGCTG ATTCGTTCTC TACGATGTCA CGCGTTAAGT 2701 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt