Construct: ORF TRCN0000481620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006874.1_s317c1
- Derived from:
- ccsbBroadEn_02476
- DNA Barcode:
- AGCAGTGCTCCTAATACATTCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNLY (10578)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481620
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10578 | GNLY | granulysin | NM_006433.5 | 100% | 100% | |
| 2 | human | 10578 | GNLY | granulysin | NM_012483.4 | 89.1% | 88.2% | 1_1delAins40;5_5delAinsGCAACCC |
| 3 | human | 10578 | GNLY | granulysin | NM_001302758.2 | 84.3% | 84.3% | 51_131del |
| 4 | human | 10578 | GNLY | granulysin | XM_005264085.2 | 61.9% | 53.6% | (many diffs) |
| 5 | human | 10578 | GNLY | granulysin | XM_005264084.2 | 54.2% | 46.6% | (many diffs) |
| 6 | human | 10578 | GNLY | granulysin | XM_005264087.2 | 53.7% | 44.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tacctgggcc ctcctgctcc ttgcagccat gctcctgggc aacccaggtc 121 tggtcttctc tcgtctgagc cctgagtact acgacctggC AAGAGCCCAC CTGCGTGATG 181 AGGAGAAATC CTGCCCGTGC CTGGCCCAGG AGGGCCCCCA GGGTGACCTG TTGACCAAAA 241 CACAGGAGCT GGGCCGTGAC TACAGGACCT GTCTGACGAT AGTCCAAAAA CTGAAGAAGA 301 TGGTGGATAA GCCCACCCAG AGAAGTGTTT CCAATGCTGC GACCCGGGTG TGTAGGACGG 361 GGAGGTCACG ATGGCGCGAC GTCTGCAGAA ATTTCATGAG GAGGTATCAG TCTAGAGTTA 421 CCCAGGGCCT CGTGGCCGGA GAAACTGCCC AGCAGATCTG TGAGGACCTC AGGTTGTGTA 481 TACCTTCTAC AGGTCCCCTC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGCA GTGCTCCTAA 661 TACATTCGGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt