Construct: ORF TRCN0000481629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002754.2_s317c1
- Derived from:
- ccsbBroadEn_13947
- DNA Barcode:
- ACGTGTCAGTTCTCCCCGGGCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL38 (6169)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6169 | RPL38 | ribosomal protein L38 | NM_000999.4 | 100% | 100% | |
| 2 | human | 6169 | RPL38 | ribosomal protein L38 | NM_001035258.2 | 100% | 100% | |
| 3 | mouse | 67671 | Rpl38 | ribosomal protein L38 | NM_001048057.1 | 89.5% | 98.5% | (many diffs) |
| 4 | mouse | 67671 | Rpl38 | ribosomal protein L38 | NM_001048058.1 | 89.5% | 98.5% | (many diffs) |
| 5 | mouse | 67671 | Rpl38 | ribosomal protein L38 | NM_023372.2 | 89.5% | 98.5% | (many diffs) |
| 6 | mouse | 67671 | Rpl38 | ribosomal protein L38 | XM_006534009.2 | 89.5% | 98.5% | (many diffs) |
| 7 | mouse | 67671 | Rpl38 | ribosomal protein L38 | XM_017314720.1 | 89.5% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 273
- ORF end:
- 483
- ORF length:
- 210
- Sequence:
-
1 CCTCGATTAG TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA 61 TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT 121 GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC 181 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 241 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGCCTCG GAAAATTGAG GAAATCAAGG 301 ACTTCCTGCT CACAGCCCGA CGAAAGGATG CCAAATCTGT CAAGATCAAG AAAAATAAGG 361 ACAACGTGAA GTTTAAAGTT CGATGCAGCA GATACCTTTA CACCCTGGTC ATCACTGACA 421 AAGAGAAGGC AGAGAAACTG AAGCAGTCCC TGCCCCCCGG TTTGGCAGTG AAGGAACTGA 481 AATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGAAC GTGTCAGTTC TCCCCGGGCC TCACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt