Construct: ORF TRCN0000481632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013716.1_s317c1
- Derived from:
- ccsbBroadEn_08465
- DNA Barcode:
- AGCTTTTCTCGGTGTTGGCAAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC52A1 (55065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55065 | SLC52A1 | solute carrier family 52 me... | NM_001104577.1 | 99.8% | 99.5% | 209A>G;812C>T |
| 2 | human | 55065 | SLC52A1 | solute carrier family 52 me... | NM_017986.4 | 99.8% | 99.5% | 209A>G;812C>T |
| 3 | human | 55065 | SLC52A1 | solute carrier family 52 me... | XM_011523951.2 | 99.8% | 99.5% | 209A>G;812C>T |
| 4 | human | 79581 | SLC52A2 | solute carrier family 52 me... | NM_001253815.2 | 86.7% | 86.1% | (many diffs) |
| 5 | human | 79581 | SLC52A2 | solute carrier family 52 me... | NM_001253816.2 | 86.7% | 86.1% | (many diffs) |
| 6 | human | 79581 | SLC52A2 | solute carrier family 52 me... | NM_001363118.2 | 86.7% | 86.1% | (many diffs) |
| 7 | human | 79581 | SLC52A2 | solute carrier family 52 me... | NM_001363120.2 | 86.7% | 86.1% | (many diffs) |
| 8 | human | 79581 | SLC52A2 | solute carrier family 52 me... | NM_001363121.2 | 86.7% | 86.1% | (many diffs) |
| 9 | human | 79581 | SLC52A2 | solute carrier family 52 me... | NM_024531.5 | 86.7% | 86.1% | (many diffs) |
| 10 | human | 79581 | SLC52A2 | solute carrier family 52 me... | XM_011517300.2 | 69.1% | 68.5% | (many diffs) |
| 11 | human | 79581 | SLC52A2 | solute carrier family 52 me... | NM_001363122.2 | 54.1% | 53.1% | (many diffs) |
| 12 | human | 79581 | SLC52A2 | solute carrier family 52 me... | XM_017013821.1 | 54.1% | 53.1% | (many diffs) |
| 13 | human | 79581 | SLC52A2 | solute carrier family 52 me... | XM_017013822.1 | 54.1% | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1410
- ORF length:
- 1344
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcacccacg ctgggccgtc tggtgctgac ccacctgctg gtggcccttt 121 ttggcatggg ctcctgggct gctgtgaacg ggatctgggt ggagctgcct gtggtggtaa 181 aagaccttcc agagggttgg agcctcccct catacctctc tgtggttgtg gcgctgggaa 241 acctgggtct gctggtggtg accctgtgga ggcggctggc cccgggcaag ggcgagcagg 301 tccccatcca ggtggtacag gtgctgagtg tagtgggcac agccctgctg gcccctctgt 361 ggcaccacgt ggccccagtg gcagggcagc tccactctgt ggccttccta actctggcct 421 tggtgttggc aatggcctgt tgtacctcta atgtcacttt cctgcccttc ctgagccacc 481 tgccacctcc tttcttacgg tctttcttcc tgggtcaggg tctcagtgcc ctactcccct 541 gtgtgctggc cctagtgcaa ggtgtgggcc gcctcgagtg cccaccagcg cccaccaatg 601 gcacctctgg gcctcccctc gacttccctg agcgttttcc tgccagcacc ttcttctggg 661 cactgactgc ccttctggtc acttcagctg ccgccttccg gggtctcctg ttgctgttgc 721 catcactacc ctctgtaacc acagggggct cagggcctga acttcaactg ggatccccag 781 gagcagagga ggaagagaag gaggaagaag aggctttgcc attgcaggag ccaccgagcc 841 aggcagcagg caccatccct ggcccagacc ctgaggtcca tcagctgttc tcagcccatg 901 gtgccttcct gctgggcctg atggCCTTCA CCAGTGCCGT GACCAATGGC GTGCTGCCTT 961 CTGTGCAGAG CTTTTCCTGT TTGCCCTATG GGCGCCTGGC CTACCACCTG GCTGTGGTGC 1021 TGGGCAGTGC CGCCAACCCC CTTGCCTGCT TCCTGGCCAT GGGCGTGCTG TGCAGGTCCC 1081 TGGCAGGGCT GGTTGGTCTT TCTCTGCTGG GCATGCTCTT TGGGGCCTAC CTGATGGCAC 1141 TGGCAATCCT GAGCCCCTGC CCACCCCTGG TGGGCACCAC TGCAGGGGTG GTCCTTGTGG 1201 TGCTGTCGTG GGTGCTGTGT CTGTGTGTGT TCTCATATGT GAAGGTGGCT GCAAGCTCCC 1261 TGCTGCATGG TGGGGGTCGG CCGGCATTGC TGGCAGCTGG TGTGGCCATC CAAGTGGGCT 1321 CCCTGCTTGG TGCCGGTGCC ATGTTCCCTC CCACCAGCAT CTACCACGTG TTTCAAAGCA 1381 GAAAGGACTG TGTAGACCCC TGTGGCCCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1441 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1501 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGCTT 1561 TTCTCGGTGT TGGCAAGACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1621 tgaaagatt