Construct: ORF TRCN0000481640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018422.1_s317c1
- Derived from:
- ccsbBroadEn_06281
- DNA Barcode:
- AGCCCCATATTGGCCGGGGCCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLB1 (2720)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2720 | GLB1 | galactosidase beta 1 | NM_000404.4 | 99.8% | 99.7% | 29C>T;34T>C;1561T>C |
| 2 | human | 2720 | GLB1 | galactosidase beta 1 | NM_001079811.2 | 95.5% | 95.5% | 0_1ins90 |
| 3 | human | 2720 | GLB1 | galactosidase beta 1 | NM_001317040.1 | 93.2% | 93.2% | 29C>T;34T>C;76_219del |
| 4 | human | 2720 | GLB1 | galactosidase beta 1 | NM_001135602.2 | 80.5% | 77.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2097
- ORF length:
- 2031
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggggttcctg gttcgcatcc tccttctgct gctggttctg ctgcttctgg 121 gccctacgcg cggcttgcgc aatgccaccc agaggatgtt tgaaattgac tatagccggg 181 actccttcct caaggatggc cagccatttc gctacatctc aggaagcatt cactactccc 241 gtgtgccccg cttctactgg aaggaccggc tgctgaagat gaagatggct gggctgaacg 301 ccatccagac gtatgtgccc tggaactttc atgagccctg gccaggacag taccagtttt 361 ctgaggacca tgatgtggaa tattttcttc ggctggctca tgagctggga ctgctggtta 421 tcctgaggcc cgggccctac atctgtgcag agtgggaaat gggaggatta cctgcttggc 481 tgctagagaa agagtctatt cttctccgct cctccgaccc agattacctg gcagctgtgg 541 acaagtggtt gggagtcctt ctgcccaaga tgaagcctct cctctatcag aatggagggc 601 cagttataac agtgcaggtt gaaaatgaat atggcagcta ctttgcctgt gattttgact 661 acctgcgctt cctgcagaag cgctttcgcc accatctggg ggatgatgtg gttctgttta 721 ccactgatgg agcacataaa acattcctga aatgtggggc cctgcagggc ctctacacca 781 cggtggactt tggaacaggc agcaacatca cagatgcttt cctaagccag aggaagtgtg 841 agcccaaagg acccttgatc aattctgaat tctatactgg ctggctagat cactggggcc 901 aacctcactc cacaatcaag accgaagcag tggcttcctc cctctatgat atacttgccc 961 gtggggcgag tgtgaacttg tacatgttta taggtgggac caattttgcc tattggaatg 1021 gggccaactc accctatgca gcacagccca ccagctacga ctatgatgcc ccactgagtg 1081 aggctgggga cctcactgag aagtattttg ctctgcgaaa catcatccag aagtttgaaa 1141 aagtaccaga aggtcctatc cctccatcta caccaaagtt tgcatatgga aaggtcactt 1201 tggaaaagtt aaagacagtg ggagcagctc tggacattct gtgtccctct gggcccatca 1261 aaagccttta tcccttgaca tttatccagg tgaaacagca ttatgggttt gtgctgtacc 1321 ggacaacact tcctcaagat tgcagcaacc cagcacctct ctcttcaccc ctcaatggag 1381 tccacgatcg agcatatgtt gctgtggatg ggatccccca gggagtcctt gagcgaaaca 1441 atgtgatcac tctgaacata acagggaaag ctggagccac tctggacctt ctggtagaga 1501 acatgggacg tgtgaactat ggtgcatata tcaacgattt taagggtttg gtttctaacc 1561 tgactctcag ttccaatatc ctcacggact ggacgatctt tcCACTGGAC ACTGAGGATG 1621 CAGTGCGCAG CCACCTGGGG GGCTGGGGAC ACCGTGACAG TGGCCACCAT GATGAAGCCT 1681 GGGCCCACAA CTCATCCAAC TACACGCTCC CGGCCTTTTA TATGGGGAAC TTCTCCATTC 1741 CCAGTGGGAT CCCAGACTTG CCCCAGGACA CCTTTATCCA GTTTCCTGGA TGGACCAAGG 1801 GCCAGGTCTG GATTAATGGC TTTAACCTTG GCCGCTATTG GCCAGCCCGG GGCCCTCAGT 1861 TGACCTTGTT TGTGCCCCAG CACATCCTGA TGACCTCGGC CCCAAACACC ATCACCGTGC 1921 TGGAACTGGA GTGGGCACCC TGCAGCAGTG ATGATCCAGA ACTATGTGCT GTGACGTTCG 1981 TGGACAGGCC AGTTATTGGC TCATCTGTGA CCTACGATCA TCCCTCCAAA CCTGTTGAAA 2041 AAAGACTCAT GCCCCCACCC CCGCAAAAAA ACAAAGATTC ATGGCTGGAC CATGTATGCC 2101 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2161 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2221 ATATATCTTG TGGAAAGGAC GAAGCCCCAT ATTGGCCGGG GCCCCTACGC GTTAAGTCga 2281 caatcaacct ctggattaca aaatttgtga aagatt