Construct: ORF TRCN0000481655
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005792.1_s317c1
- Derived from:
- ccsbBroadEn_11188
- DNA Barcode:
- CGGTATAGCCGAATACCATTACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIAL1 (7073)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481655
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NM_001323964.1 | 100% | 100% | |
| 2 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NM_001323965.1 | 100% | 100% | |
| 3 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NM_001323967.1 | 100% | 100% | |
| 4 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NM_001323969.1 | 75% | 74.7% | 1_251del;254delC |
| 5 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NM_001323970.1 | 71.3% | 71.1% | 1_302del;305delC |
| 6 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | XM_024448151.1 | 71.3% | 71.1% | 1_302del;305delC |
| 7 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NM_001323968.1 | 70% | 69.4% | 1_302del;305delC;936_956del |
| 8 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NM_003252.3 | 67.2% | 66.9% | 1_368del;371delC |
| 9 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NM_001033925.1 | 64.2% | 64% | 1_419del;422delC |
| 10 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NR_136661.1 | 17.9% | (many diffs) | |
| 11 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NR_136663.1 | 17.6% | (many diffs) | |
| 12 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | NR_136662.1 | 17.4% | 1_1052del;1543_1871del;2138_4340del | |
| 13 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | XR_945808.2 | 13% | (many diffs) | |
| 14 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | XR_001747195.2 | 12.7% | (many diffs) | |
| 15 | human | 7073 | TIAL1 | TIA1 cytotoxic granule asso... | XR_001747194.2 | 12.6% | (many diffs) | |
| 16 | mouse | 21843 | Tial1 | Tia1 cytotoxic granule-asso... | XM_006507575.3 | 92.7% | 98.8% | (many diffs) |
| 17 | mouse | 21843 | Tial1 | Tia1 cytotoxic granule-asso... | XM_017322108.1 | 92.7% | 98.8% | (many diffs) |
| 18 | mouse | 21843 | Tial1 | Tia1 cytotoxic granule-asso... | XM_017322109.1 | 92.7% | 98.8% | (many diffs) |
| 19 | mouse | 21843 | Tial1 | Tia1 cytotoxic granule-asso... | NM_001347641.1 | 69.5% | 73.8% | (many diffs) |
| 20 | mouse | 21843 | Tial1 | Tia1 cytotoxic granule-asso... | XM_006507571.2 | 66.1% | 70.2% | (many diffs) |
| 21 | mouse | 21843 | Tial1 | Tia1 cytotoxic granule-asso... | NM_001347640.1 | 62.3% | 66.1% | (many diffs) |
| 22 | mouse | 21843 | Tial1 | Tia1 cytotoxic granule-asso... | NM_009383.2 | 59.6% | 63.2% | (many diffs) |
| 23 | mouse | 21843 | Tial1 | Tia1 cytotoxic granule-asso... | XR_378231.3 | 35.3% | (many diffs) | |
| 24 | mouse | 21843 | Tial1 | Tia1 cytotoxic granule-asso... | XR_378230.3 | 33.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 825
- ORF length:
- 756
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgcccgg gtagttaaag acatggcaac tggaaaatcc aaaggctatg 121 gttttgtatc tttttataac aaactggatg cagaaaatgc gattgtgcat atgggcggtc 181 agtggttggg tggtcgtcaa atccgaacca attgggccac tcgtaaacca cctgcaccta 241 aaagtacaca agaaaacaac actaagcagt tgagatttga agatgtagta aaccagtcaa 301 gtccaaaaaa ttgtactgtg tactgtggag gaattgcgtc tgggttaaca gatcagctta 361 tgagacagac attctcacca tttggacaAA TTATGGAAAT AAGAGTTTTC CCAGAAAAGG 421 GCTATTCATT TGTCAGATTT TCAACCCATG AAAGTGCAGC CCATGCCATT GTTTCGGTGA 481 ACGGTACTAC GATTGAAGGA CATGTGGTTA AATGCTATTG GGGTAAAGAA TCTCCTGATA 541 TGACTAAAAA CTTCCAACAG GTTGACTATA GTCAATGGGG CCAATGGAGC CAAGTGTATG 601 GAAACCCACA ACAGTATGGA CAGTATATGG CAAATGGGTG GCAAGTACCG CCTTATGGAG 661 TATACGGGCA ACCATGGAAT CAACAAGGAT TTGGAGTAGA TCAATCACCT TCTGCTGCTT 721 GGATGGGTGG ATTTGGTGCT CAGCCTCCCC AAGGACAAGC TCCTCCCCCT GTAATACCTC 781 CTCCTAACCA AGCCGGATAT GGTATGGCAA GTTACCAAAC ACAGTTGCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA CGGTATAGCC GAATACCATT ACCGACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt