Construct: ORF TRCN0000487697
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020312.1_s317c1
- DNA Barcode:
- CAAACTTCGAGTGTAATCTAATCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- VRK3 (51231)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487697
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | NM_016440.4 | 100% | 100% | |
| 2 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XM_005258971.4 | 100% | 100% | |
| 3 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XM_006723237.3 | 100% | 100% | |
| 4 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | NM_001025778.1 | 89.4% | 89.4% | 136_137ins150 |
| 5 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XM_005258972.4 | 89.4% | 89.4% | 136_137ins150 |
| 6 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XM_006723238.4 | 89.4% | 89.4% | 136_137ins150 |
| 7 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | NM_001308420.2 | 86.6% | 84.9% | (many diffs) |
| 8 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_001753695.2 | 64.1% | 1_91del;1309_1679del;1885_2215del | |
| 9 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_001753696.1 | 61.6% | 1_181del;1399_1769del;1975_2305del | |
| 10 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_001753698.2 | 61.1% | (many diffs) | |
| 11 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_001753697.2 | 53.4% | (many diffs) | |
| 12 | human | 51231 | VRK3 | VRK serine/threonine kinase 3 | XR_002958320.1 | 47.1% | 1_91del;1309_2483del;2689_3019del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1494
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatctcc ttctgtccag actgtggcaa aagtatccaa gcggcattca 121 aattctgccc ctactgtgga aattctttgc ctgtagagga gcatgtaggg tcccagacct 181 ttgtcaatcc acatgtgtca tccttccaag gctcaaagag agggctgaac tccagttttg 241 aaacctctcc taagaaagtg aaatggtcca gcaccgtcac ctctccccga ttatccctct 301 tctcagatgg tgacagttct gagtctgaag atactctgag ttcctctgag agatccaaag 361 gctccgggag cagaccccca acccccaaaa gcagccctca gaagaccagg aagagccctc 421 aggtgaccag gggtagccct cagaagacca gctgtagccc tcagaagacc aggcagagcc 481 ctcagacgct gaagcggagc cgagtgacca cctcacttga agctttgccc acagggacag 541 tgctgacaga caagagtggg cgacagtgga agctgaagtc cttccagacc agggacaacc 601 agggcattct ctatgaagct gcacccacct ccaccctcac ctgtgactca ggaccacaga 661 agcaaaagtt ctcactcaaa ctggatgcca aggatgggcg cttgttcaat gagcagaact 721 tcttccagcg ggccgccaag cctctgcaag tcaacaagtg gaagaagctg tactcgaccc 781 cactgctggc catccctacc tgcatgggtt tcggtgttca ccaggacaaa tacaggttct 841 tggtgttacc cagcctgggg aggagccttc agtcggccct ggatgtcagc ccaaagcatg 901 tgctgtcaga gaggtctgtg ctgcaggtgg cctgccggct gctggatgcc ctggagttcc 961 tccatgagaa tgagtatgtt catggaaatg tgacagctga aaatatcttt gtggatccag 1021 aggaccagag tcaggtgact ttggcaggct atggcttcgc cttccgctat tgcccaagtg 1081 gcaaacacgt ggcctacgtg gaaggcagca ggagccctca cgagggggac cttgagttca 1141 ttagcatgga cctgcacaag ggatgcgggc cctcccgccg cagcgacctc cagagcctgg 1201 gctactgcat gctgaagtgg ctctacgggt ttctgccatg gacaaattgc cttcccaaca 1261 ctgaggacat catgaagcaa aaacagaagt ttgttgataa gccggggccc ttcgtgggac 1321 cctgcggtca ctggatcagg ccctcagaga ccctgcagaa gtacctgaag gtggtgatgg 1381 ccctcacgta tgaggagaag ccgcccTACG CCATGCTGAG GAACAACCTA GAAGCTTTGC 1441 TGCAGGATCT GCGTGTGTCT CCATATGACC CCATTGGCCT CCCGATGGTG CCCTGAGACC 1501 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1561 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1621 ATATATCTTG TGGAAAGGAC GACAAACTTC GAGTGTAATC TAATCCACGC GTTAAGTCga 1681 caatcaacct ctggattaca aaatttgtga aagatt