Construct: ORF TRCN0000487724
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021020.1_s317c1
- DNA Barcode:
- TGATACTAGCCGTCTCTTTTCCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CALCR (799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487724
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 799 | CALCR | calcitonin receptor | NM_001164738.1 | 100% | 100% | |
| 2 | human | 799 | CALCR | calcitonin receptor | NM_001742.4 | 100% | 100% | |
| 3 | human | 799 | CALCR | calcitonin receptor | NM_001164737.2 | 96.7% | 96.7% | 522_569del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1494
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaggttc acatttacaa gccggtgctt ggcactgttt cttcttctaa 121 atcacccaac cccaattctt cctgcctttt caaatcaaac ctatccaaca atagagccca 181 agccatttct ttacgtcgta ggacgaaaga agatgatgga tgcacagtac aaatgctatg 241 accgaatgca gcagttaccc gcataccaag gagaaggtcc atattgcaat cgcacctggg 301 atggatggct gtgctgggat gacacaccgg ctggagtatt gtcctatcag ttctgcccag 361 attattttcc ggattttgat ccatcagaaa aggttacaaa atactgtgat gaaaaaggtg 421 tttggtttaa acatcctgaa aacaatcgaa cctggtccaa ctatactatg tgcaatgctt 481 tcactcctga gaaactgaag aatgcatatg ttctgtacta tttggctatt gtgggtcatt 541 ctttgtcaat tttcacccta gtgatttccc tggggatttt cgtgtttttc aggagccttg 601 gctgccaaag ggtaaccctg cacaagaaca tgtttcttac ttacattctg aattctatga 661 ttatcatcat ccacctggtt gaagtagtac ccaatggaga gctcgtgcga agggacccgg 721 tgagctgcaa gattttgcat tttttccacc agtacatgat ggcctgcaac tatttctgga 781 tgctctgtga agggatctat cttcatacac tcattgtcgt ggctgtgttt actgagaagc 841 aacgcttgcg gtggtattat ctcttgggct gggggttccc gctggtgcca accactatcc 901 atgctattac cagggccgtg tacttcaatg acaactgctg gctgagtgtg gaaacccatt 961 tgctttacat aatccatgga cctgtcatgg cggcacttgt ggtcaatttc ttctttttgc 1021 tcaacattgt ccgggtgctt gtgaccaaaa tgagggaaac ccatgaggcg gaatcccaca 1081 tgtacctgaa ggctgtgaag gccaccatga tccttgtgcc cctgctggga atccagtttg 1141 tcgtctttcc ctggagacct tccaacaaga tgcttggGAA GATATATGAT TACGTGATGC 1201 ACTCTCTGAT TCATTTCCAG GGCTTCTTTG TTGCGACCAT CTACTGCTTC TGCAACAATG 1261 AGGTCCAAAC CACCGTGAAG CGCCAATGGG CCCAATTCAA AATTCAGTGG AACCAGCGTT 1321 GGGGGAGGCG CCCCTCCAAC CGCTCTGCTC GCGCTGCAGC CGCTGCTGCG GAGGCTGGCG 1381 ACATCCCAAT TTACATCTGC CATCAGGAGC TGAGGAATGA ACCAGCCAAC AACCAAGGCG 1441 AGGAGAGTGC TGAGATCATC CCTTTGAATA TCATAGAGCA AGAGTCATCT GCTTAGAACC 1501 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1561 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1621 ATATATCTTG TGGAAAGGAC GATGATACTA GCCGTCTCTT TTCCAGACGC GTTAAGTCga 1681 caatcaacct ctggattaca aaatttgtga aagatt