Construct: ORF TRCN0000487727
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021044.1_s317c1
- DNA Barcode:
- GCCGCTCAAACTCAGCGAACCATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PAQR8 (85315)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487727
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85315 | PAQR8 | progestin and adipoQ recept... | NM_133367.5 | 99.8% | 99.4% | 53A>G;598C>T |
| 2 | mouse | 74229 | Paqr8 | progestin and adipoQ recept... | NM_028829.3 | 86.4% | 93.5% | (many diffs) |
| 3 | mouse | 74229 | Paqr8 | progestin and adipoQ recept... | XM_006495577.2 | 86.4% | 93.5% | (many diffs) |
| 4 | mouse | 74229 | Paqr8 | progestin and adipoQ recept... | XM_006495578.3 | 86.4% | 93.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1134
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacgacc gccatcttgg agcgcctgag caccctgtcg gtcagcgggc 121 agcggctgcg ccgcctgccc aagatcctgg aggatgggct tcccaagatg ccttgcactg 181 tcccagaaac ggatgtgccc cagctcttcc gggagcctta catccgcacc ggctaccgcc 241 ccacggggca cgagtggcgc tactacttct tcagcctctt tcagaaacac aacgaggtgg 301 tcaacgtctg gacccattta ctggcagccc tggccgtcct cttgcgattc tgggcctttg 361 ccgaggctga ggccttgcca tgggcgtcta cccactccct gcctctgctc ctcttcatcc 421 tgtcgtcaat cacttacctc acctgcagcc ttctggccca cctgctgcag tccaagtcag 481 agctctccca ctacaccttc tactttgtgg actatgttgg cgtgagcgtt taccaatatg 541 gcagtgcttt ggctcatttc ttctacagct ctgaccaggc ctggtatgac cggttctggc 601 ttttcttctt gccagcagct gccttctgtg gctggttatc ttgtgctggc tgttgctatg 661 ccaaatattg ttaccggagg ccttatccag tcatgaggaa gatctgtcaa gtggtgccag 721 caggtctggc ttttatccta gacatcagcc ctgtggcaca ccgtgtggcg ctctgtcacc 781 tggctggctg ccaggagcaa gcagcctggt accacaccct ccagatcctc ttcttcctgg 841 ttagcgctta tttcttctcc tgccccgtgc cTGAGAAGTA CTTCCCGGGT TCCTGTGACA 901 TCGTGGGCCA TGGGCATCAG ATCTTCCATG CATTTCTGTC CATCTGTACG CTCTCCCAGC 961 TGGAGGCCAT CCTCCTGGAC TACCAGGGGC GGCAGGAGAT CTTCCTGCAG CGCCATGGAC 1021 CCCTATCTGT CCACATGGCC TGCCTCTCCT TCTTCTTCCT GGCTGCCTGC AGTGCTGCCA 1081 CCGCAGCCCT TCTGAGGCAC AAAGTCAAGG CCAGACTGAC CAAGAAAGAT TCCTAGAACC 1141 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GAGCCGCTCA AACTCAGCGA ACCATCACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt