Construct: ORF TRCN0000487740
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021327.1_s317c1
- DNA Barcode:
- TTATAAGGGGAAATGATGGGGATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BRK1 (55845)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487740
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55845 | BRK1 | BRICK1 subunit of SCAR/WAVE... | NM_018462.5 | 100% | 100% | |
| 2 | mouse | 101314 | Brk1 | BRICK1, SCAR/WAVE actin-nuc... | NM_133937.1 | 94.6% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 318
- ORF length:
- 225
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGCAGGCA CCATGGCGGG ACAGGAGGAT CCGGTGCAGC 121 GGGAGATTCA CCAGGACTGG GCTAACCGGG AGTACATTGA GATAATCACC AGCAGCATCA 181 AGAAAATCGC AGACTTTCTC AACTCGTTCG ATATGTCTTG TCGTTCAAGA CTTGCAACAC 241 TAAACGAGAA ATTGACAGCC CTTGAACGGA GAATAGAGTA CATTGAAGCT CGGGTGACAA 301 AAGGTGAGAC ACTCACCTAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 361 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 421 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTAT AAGGGGAAAT 481 GATGGGGATC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt