Transcript: Mouse NM_133937.1

Mus musculus BRICK1, SCAR/WAVE actin-nucleating complex subunit (Brk1), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Brk1 (101314)
Length:
1110
CDS:
8..235

Additional Resources:

NCBI RefSeq record:
NM_133937.1
NBCI Gene record:
Brk1 (101314)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133937.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000137079 CGATATGTCTTGTCGTTCAAG pLKO.1 124 CDS 100% 4.950 6.930 N BRK1 n/a
2 TRCN0000202277 GAACCGGGAGTACATTGAGAT pLKO.1 58 CDS 100% 4.950 6.930 N Brk1 n/a
3 TRCN0000314476 GAACCGGGAGTACATTGAGAT pLKO_005 58 CDS 100% 4.950 6.930 N Brk1 n/a
4 TRCN0000191856 GAACTACTTGTCAATGTGTTT pLKO.1 486 3UTR 100% 4.950 6.930 N Brk1 n/a
5 TRCN0000314418 GAACTACTTGTCAATGTGTTT pLKO_005 486 3UTR 100% 4.950 6.930 N Brk1 n/a
6 TRCN0000200527 CGGAGAATAGAGTACATTGAA pLKO.1 182 CDS 100% 5.625 4.500 N Brk1 n/a
7 TRCN0000314417 CGGAGAATAGAGTACATTGAA pLKO_005 182 CDS 100% 5.625 4.500 N Brk1 n/a
8 TRCN0000136735 GAGAAATTGACAGCCCTTGAA pLKO.1 161 CDS 100% 4.950 3.960 N BRK1 n/a
9 TRCN0000191458 CGCAACACTAAATGAGAAATT pLKO.1 148 CDS 100% 13.200 9.240 N Brk1 n/a
10 TRCN0000314477 CGCAACACTAAATGAGAAATT pLKO_005 148 CDS 100% 13.200 9.240 N Brk1 n/a
11 TRCN0000191796 GAACGGAGAATAGAGTACATT pLKO.1 179 CDS 100% 5.625 3.938 N Brk1 n/a
12 TRCN0000134951 GAATAGAGTACATTGAAGCTC pLKO.1 186 CDS 100% 2.640 2.112 N BRK1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133937.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03664 pDONR223 100% 94.6% 98.6% None (many diffs) n/a
2 ccsbBroad304_03664 pLX_304 0% 94.6% 98.6% V5 (many diffs) n/a
3 TRCN0000474926 GATTCCGCGCAGTGTCTGTAAGAA pLX_317 100% 94.6% 98.6% V5 (many diffs) n/a
4 TRCN0000487740 TTATAAGGGGAAATGATGGGGATC pLX_317 100% 94.6% 98.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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