Construct: ORF TRCN0000487741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021034.1_s317c1
- DNA Barcode:
- GGCAGTTTCAGTCTATCACAGTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRM3 (2913)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487741
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2913 | GRM3 | glutamate metabotropic rece... | NM_000840.2 | 100% | 100% | |
| 2 | human | 2913 | GRM3 | glutamate metabotropic rece... | NM_001363522.1 | 58.6% | 52.8% | 1324_1325ins1067;1571_1611del |
| 3 | human | 2913 | GRM3 | glutamate metabotropic rece... | XM_017012073.2 | 53.2% | 50.3% | (many diffs) |
| 4 | mouse | 108069 | Grm3 | glutamate receptor, metabot... | NM_181850.2 | 89.6% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2709
- ORF length:
- 2637
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagatg ttgacaagac tgcaagttct taccttagct ttgttttcaa 121 agggattttt actctcttta ggggaccata actttctaag gagagagatt aaaatagaag 181 gtgaccttgt tttagggggc ctgtttccta ttaacgaaaa aggcactgga actgaagaat 241 gtgggcgaat caatgaagac cgagggattc aacgcctgga agccatgttg tttgctattg 301 atgaaatcaa caaagatgat tacttgctac caggagtgaa gttgggtgtt cacattttgg 361 atacatgttc aagggatacc tatgcattgg agcaatcact ggagtttgtc agggcatctt 421 tgacaaaagt ggatgaagct gagtatatgt gtcctgatgg atcctatgcc attcaagaaa 481 acatcccact tctcattgca ggggtcattg gtggctctta tagcagtgtt tccatacagg 541 tggcaaacct gctgcggctc ttccagatcc ctcagatcag ctacgcatcc accagcgcca 601 aactcagtga taagtcgcgc tatgattact ttgccaggac cgtgcccccc gacttctacc 661 aggccaaagc catggctgag atcttgcgct tcttcaactg gacctacgtg tccacagtag 721 cctccgaggg tgattacggg gagacaggga tcgaggcctt cgagcaggaa gcccgcctgc 781 gcaacatctg catcgctacg gcggagaagg tgggccgctc caacatccgc aagtcctacg 841 acagcgtgat ccgagaactg ttgcagaagc ccaacgcgcg cgtcgtggtc ctcttcatgc 901 gcagcgacga ctcgcgggag ctcattgcag ccgccagccg cgccaatgcc tccttcacct 961 gggtggccag cgacggctgg ggcgcgcagg agagcatcat caagggcagc gagcatgtgg 1021 cctacggcgc catcaccctg gagctggcct cccagcctgt ccgccagttc gaccgctact 1081 tccagagcct caacccctac aacaaccacc gcaacccctg gttccgggac ttctgggagc 1141 aaaagtttca gtgcagcctc cagaacaaac gcaaccacag gcgcgtctgc gacaagcacc 1201 tggccatcga cagcagcaac tacgagcaag agtccaagat catgtttgtg gtgaacgcgg 1261 tgtatgccat ggcccacgct ttgcacaaaa tgcagcgcac cctctgtccc aacactacca 1321 agctttgtga tgctatgaag atcctggatg ggaagaagtt gtacaaggat tacttgctga 1381 aaatcaactt cacggctcca ttcaacccaa ataaagatgc agatagcata gtcaagtttg 1441 acacttttgg agatggaatg gggcgataca acgtgttcaa tttccaaaat gtaggtggaa 1501 agtattccta cttgaaagtt ggtcactggg cagaaacctt atcgctagat gtcaactcta 1561 tccactggtc ccggaactca gtccccactt cccagtgcag cgacccctgt gcccccaatg 1621 aaatgaagaa tatgcaacca ggggatgtct gctgctggat ttgcatcccc tgtgaaccct 1681 acgaatacct ggctgatgag tttacctgta tggattgtgg gtctggacag tggcccactg 1741 cagacctaac tggatgctat gaccttcctg aggactacat caggtgggaa gacgcctggg 1801 ccattggccc agtcaccatt gcctgtctgg gttttatgtg tacatgcatg gttgtaactg 1861 tttttatcaa gcacaacaac acacccttgg tcaaagcatc gggccgagaa ctctgctaca 1921 tcttattgtt tggggttggc ctgtcatact gcatgacatt cttcttcatt gccaagccat 1981 caccagtcat ctgtgcattg cgccgactcg ggctggggag ttccttcgct atctgttact 2041 cagccctgct gaccaagaca aactgcattg cccgcatctt cgatggggtc aagaatggcg 2101 ctcagaggcc aaaattcatc agccccagtt ctcaggtttt catctgcctg ggtctgatcc 2161 tggtgcaaat tgtgatggtg tctgtgtggc tcatcctgga ggccccaggc accaggaggt 2221 atacccttgc agagaagcgg gaaacagtca tcctaaaatg caatgtcaaa gattccagca 2281 tgttgatctc tcttacctac gatgtgatcc tggtgatctt atgcactgtg tacgccttca 2341 aaacgcggaa gtgcccagaa aatttcaacg aagctaagtt cataggtttt accatgtaca 2401 ccacgtgcat catctggttg gCCTTCCTCC CTATATTTTA TGTGACATCA AGTGACTACA 2461 GAGTGCAGAC GACAACCATG TGCATCTCTG TCAGCCTGAG TGGCTTTGTG GTCTTGGGCT 2521 GTTTGTTTGC ACCCAAGGTT CACATCATCC TGTTTCAACC CCAGAAGAAT GTTGTCACAC 2581 ACAGACTGCA CCTCAACAGG TTCAGTGTCA GTGGAACTGG GACCACATAC TCTCAGTCCT 2641 CTGCAAGCAC GTATGTGCCA ACGGTGTGCA ATGGGCGGGA AGTCCTCGAC TCCACCACCT 2701 CATCTCTGTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2761 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2821 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGC AGTTTCAGTC TATCACAGTT 2881 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t