Construct: ORF TRCN0000487751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020344.1_s317c1
- DNA Barcode:
- TGCCCTGACGACATTAAAACGATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK17A (9263)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487751
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9263 | STK17A | serine/threonine kinase 17a | NM_004760.3 | 99.9% | 99.7% | 1084A>G |
| 2 | human | 9263 | STK17A | serine/threonine kinase 17a | XM_017012792.1 | 77.9% | 77.7% | 0_1ins273;811A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1314
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatccct ttggagaagc caggcagcgg cggctcctcc ccaggcgcca 121 cctcaggctc gggccgggca ggccggggtc tgagcgggcc gtgccggccg ccgccgccgc 181 cccaggcccg cgggctgctg acagagatac gcgccgtggt gcgcaccgag cccttccagg 241 acggctacag cctgtgcccg ggccgggagc tgggcagggg gaaatttgca gtggtgagaa 301 aatgtataaa gaaagattct gggaaagaat ttgctgcaaa gttcatgaga aaaagaagaa 361 aaggccaaga ttgtcggatg gaaataattc atgagattgc tgtacttgaa ctagcacaag 421 acaatccttg ggtcattaat ttacatgaag tttatgagac tgcatcagaa atgatcttag 481 ttctggaata tgctgctggg ggtgaaatct ttgaccagtg tgttgcagac agagaagaag 541 cctttaaaga aaaagatgtt caaagactta tgcgacagat tttagaaggt gttcactttt 601 tacacactcg tgatgtagtt catcttgatt tgaagcctca gaatattctg ttgacaagtg 661 aatctccatt gggtgacatt aagattgttg attttggcct ttcaagaata ttgaagaaca 721 gtgaagagct ccgagaaatt atgggtaccc ctgaatatgt ggctcctgaa attcttagtt 781 atgatcctat aagcatggca acagatatgt ggagcattgg agtgttaaca tatgtcatgc 841 ttacaggaat atcacctttc ttaggcaatg ataaacaaga aacattctta aacatctcac 901 agatgaattt aagttattct gaggaagaat ttgatgtttt gtctgagtcg gctgttgatt 961 tcatcaggac acttttagtt aagaaacctg aagatcgagc cactgctgaa gaatgtctaa 1021 agcacccctg gttgacacag agcagtattc aagagccttc tttcaggatg gaaaaggcac 1081 tagaagaagc aaatgccctc caagaaggtc attctgtgcc tgaaattaat tcggataccg 1141 acaaatcaga aaccgaggaa tCCATTGTAA CCGAAGAGTT AATTGTAGTT ACTTCATATA 1201 CTCTAGGACA ATGCAGACAG TCTGAAAAAG AGAAAATGGA GCAAAAGGCC ATTTCCAAAC 1261 GATTTAAATT TGAGGAACCT TTGCTACAAG AAATTCCAGG AGAATTTATC TACTGAGACC 1321 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GATGCCCTGA CGACATTAAA ACGATCACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt