Construct: ORF TRCN0000487753

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021915.1_s317c1
DNA Barcode:
GGGTAATGTACGACTTACACTGTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ERN1 (2081)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487753
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2081 ERN1 endoplasmic reticulum to nu... NM_001433.5 99.5% 100% (many diffs)
2 human 2081 ERN1 endoplasmic reticulum to nu... XM_017024347.2 95.4% 94.9% (many diffs)
3 human 2081 ERN1 endoplasmic reticulum to nu... XM_017024348.2 92% 91.6% (many diffs)
4 mouse 78943 Ern1 endoplasmic reticulum (ER) ... NM_023913.2 88.4% 92.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
96
ORF end:
3027
ORF length:
2931
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggctccgc ggccgccccc ttcaccaagc ttgccatgcc tgcacgacga ctgctgctgc
121tgctgacgct gctgctgccc ggcctcggga tttttggaag taccagcaca gtgacgcttc
181ctgaaacctt gttgtttgtg tcaacgctgg atggaagttt gcatgctgtc agcaagagga
241caggctcaat caaatggact ttaaaagaag atccagtcct gcaggtccca acacatgtgg
301aagagcctgc ctttctccca gatcctaatg atggcagcct gtatacgctt ggaagcaaga
361ataatgaagg cctgacgaaa cttcctttta ccatcccaga attggtgcag gcatccccat
421gccgaagttc agatggaatc ctctacatgg gtaaaaagca ggacatctgg tatgttattg
481acctcctgac cggagagaag cagcagactt tgtcatcggc ctttgcagat agtctctgcc
541catcaacctc tcttctgtat cttgggcgaa cagaatacac catcaccatg tacgacacca
601aaacccgaga gctccggtgg aatgccacct actttgacta tgcggcctca ctgcctgagg
661acgacgtgga ctacaagatg tcccactttg tgtccaatgg tgatgggctg gtggtgactg
721tggacagtga atctggggac gtcctgtgga tccaaaacta cgcctcccct gtggtggcct
781tttatgtctg gcagcgggag ggtctgagga aggtgatgca catcaatgtc gctgtggaga
841ccctgcgcta tctgaccttc atgtctgggg aggtggggcg catcacaaag tggaagtacc
901cgttccccaa ggagacagag gccaagagca agctgacgcc cactctgtat gttgggaaat
961actctaccag cctctatgcc tctccctcaa tggtacacga gggggttgct gtcgtgcccc
1021gcggcagcac acttcctttg ctggaagggc cccagactga tggcgtcacc atcggggaca
1081agggggagtg tgtgatcacg cccagcacgg acgtcaagtt tgatcccgga ctcaaaagca
1141agaacaagct caactacttg aggaattact ggcttctgat aggacaccat gaaaccccac
1201tgtctgcgtc taccaagatg ctggagagat ttcccaacaa tctacccaaa catcgggaaa
1261atgtgattcc tgctgattca gagaaaaaga gctttgagga agttatcaac ctggttgacc
1321agacttcaga aaacgcacct accaccgtgt ctcgggatgt ggaggagaag cccgcccatg
1381cccctgcccg gcccgaggcc cccgtggact ccatgcttaa ggacatggct accatcatcc
1441tgagcacctt cctgctgatt ggctgggtgg ccttcatcat cacctatccc ctgagcatgc
1501atcagcagca gcagctccag caccagcagt tccagaagga actggagaag atccagctcc
1561tgcagcagca gcagcagcag ctgcccttcc acccacctgg agacacggct caggacggcg
1621agctcctgga cacgtctggc ccgtactcag agagctcggg caccagcagc cccagcacgt
1681cccccagggc ctccaaccac tcgctctgct ccggcagctc tgcctccaag gctggcagca
1741gcccctccct ggaacaagac gatggagatg aggaaaccag cgtggtgata gttgggaaaa
1801tttccttctg tcccaaggat gtcctgggcc atggagctga gggcacaatt gtgtaccggg
1861gcatgtttga caaccgcgac gtggccgtga agaggatcct ccccgagtgt tttagcttcg
1921cagaccgtga ggtccagctg ttgcgagaat cggatgagca cccgaacgtg atccgctact
1981tctgcacgga gaaggaccgg caattccagt acattgccat cgagctgtgt gcagccaccc
2041tgcaagagta tgtggagcag aaggactttg cgcatctcgg cctggagccc atcaccttgc
2101tgcagcagac cacctcgggc ctggcccacc tccactccct caacatcgtt cacagagacc
2161taaagccaca caacatcctc atatccatgc ccaatgcaca cggcaagatc aaggccatga
2221tctccgactt tggcctctgc aagaagctgg cagtgggcag acacagtttc agccgccgat
2281ctggggtgcc tggcacagaa ggctggatcg ctccagagat gctgagcgaa gactgtaagg
2341agaaccctac ctacacggtg gacatctttt ctgcaggctg cgtcttttac tacgtaatct
2401ctgagggcag ccaccctttt ggcaagtccc tgcagcggca ggccaacatc ctcctgggtg
2461cctgcagcct tgactgcttg cacccagaga agcacgaaga cgtcattgca cgtgaattga
2521tagagaagat gattgcgatg gatcctcaga aacgcccctc agcgaagcac gtgctcaaac
2581acccgttctt ctggagccta gagaagcagc tccagttctt ccaggacgtg agcgacagaa
2641tagaaaagga atccctggat ggcccgatcg tgaagcagtt agagagaggc gggagagccg
2701tggtgaagat ggactggcgg gagaacatca ctgtccccct ccagacagac ctgcgtaaat
2761tcaggaccta taaaggtggt tctgtcagag atctccTCCG AGCCATGAGA AATAAGAAGC
2821ACCACTACCG GGAGCTGCCT GCAGAGGTGC GGGAGACGCT GGGGTCCCTC CCCGACGACT
2881TCGTGTGCTA CTTCACGTCT CGCTTCCCCC ACCTCCTCGC ACACACCTAC CGGGCCATGG
2941AGCTGTGCAG CCACGAGAGA CTCTTCCAGC CCTACTACTT CCACGAGCCC CCAGAGCCCC
3001AGCCCCCAGT GACTCCAGAC GCCCTCTGAT CTAGAAAGGG TGGGCGCGCC GACCCAGCTT
3061TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
3121CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
3181CTTGTGGAAA GGACGAGGGT AATGTACGAC TTACACTGTC ACGCGTTAAG TCgacaatca
3241acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.