Construct: ORF TRCN0000487757
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021047.1_s317c1
- DNA Barcode:
- CACGCCTCTGGACTTTCCCAGTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTGER3 (5733)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487757
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198716.2 | 100% | 100% | |
| 2 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198717.2 | 97.5% | 97.5% | 1077_1078ins27 |
| 3 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_001126044.2 | 94.5% | 90.4% | (many diffs) |
| 4 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198714.2 | 94.5% | 90.4% | (many diffs) |
| 5 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198719.2 | 94.5% | 90.4% | (many diffs) |
| 6 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198715.3 | 94.2% | 90% | (many diffs) |
| 7 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | XM_011541810.3 | 90.7% | 86.1% | (many diffs) |
| 8 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198718.2 | 89.1% | 86.7% | (many diffs) |
| 9 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | XR_946714.2 | 74.7% | 1_231del;1336_1428del;1447_1501del | |
| 10 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NR_028293.2 | 56.2% | (many diffs) | |
| 11 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NR_028292.2 | 55.5% | (many diffs) | |
| 12 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NR_028294.2 | 53.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1194
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaggag acccggggct acggagggga tgcccccttc tgcacccgcc 121 tcaaccactc ctacacaggc atgtgggcgc ccgagcgttc cgccgaggcg cggggcaacc 181 tcacgcgccc tccagggtct ggcgaggatt gcggatcggt gtccgtggcc ttcccgatca 241 ccatgctgct cactggtttc gtgggcaacg cactggccat gctgctcgtg tcgcgcagct 301 accggcgccg ggagagcaag cgcaagaagt ccttcctgct gtgcatcggc tggctggcgc 361 tcaccgacct ggtcgggcag cttctcacca ccccggtcgt catcgtcgtg tacctgtcca 421 agcagcgttg ggagcacatc gacccgtcgg ggcggctctg cacctttttc gggctgacca 481 tgactgtttt cgggctctcc tcgttgttca tcgccagcgc catggccgtc gagcgggcgc 541 tggccatcag ggcgccgcac tggtatgcga gccacatgaa gacgcgtgcc acccgcgctg 601 tgctgctcgg cgtgtggctg gccgtgctcg ccttcgccct gctgccggtg ctgggcgtgg 661 gccagtacac cgtccagtgg cccgggacgt ggtgcttcat cagcaccggg cgagggggca 721 acgggactag ctcttcgcat aactggggca accttttctt cgcctctgcc tttgccttcc 781 tggggctctt ggcgctgaca gtcacctttt cctgcaacct ggccaccatt aaggccctgg 841 tgtcccgctg ccgggccaag gccacggcat ctcagtccag tgcccagtgg ggccgcatca 901 cgaccgagac ggccattcag cttatgggga tcatgtgcgt gctgtcggtc tgctggtcTC 961 CGCTCCTGAT AATGATGTTG AAAATGATCT TCAATCAGAC ATCAGTTGAG CACTGCAAGA 1021 CACACACGGA GAAGCAGAAA GAATGCAACT TCTTCTTAAT AGCTGTTCGC CTGGCTTCAC 1081 TGAACCAGAT CTTGGATCCT TGGGTTTACC TGCTGTTAAG AAAGATCCTT CTTCGAAAGT 1141 TTTGCCAGAT GAGAAAAAGA AGACTCAGAG AGCAAGAGGA ATTTTGGGGA AATTAGAACC 1201 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GACACGCCTC TGGACTTTCC CAGTCAACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt