Construct: ORF TRCN0000487760
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019352.1_s317c1
- DNA Barcode:
- CGTCTAAGTGAGGGTAGGCGTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACKR4 (51554)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487760
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51554 | ACKR4 | atypical chemokine receptor 4 | NM_016557.4 | 99.9% | 99.7% | 1050_1051insG |
| 2 | human | 51554 | ACKR4 | atypical chemokine receptor 4 | NM_178445.2 | 99.9% | 99.7% | 1050_1051insG |
| 3 | mouse | 252837 | Ackr4 | atypical chemokine receptor 4 | NM_145700.2 | 83.2% | 85.1% | (many diffs) |
| 4 | mouse | 252837 | Ackr4 | atypical chemokine receptor 4 | XM_006511734.3 | 83.2% | 85.1% | (many diffs) |
| 5 | mouse | 252837 | Ackr4 | atypical chemokine receptor 4 | XM_006511735.3 | 83.2% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1128
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggct ttggaacaga accagtcaac agattattat tatgaggaaa 121 atgaaatgaa tggcacttat gactacagtc aatatgaact gatctgtatc aaagaagatg 181 tcagagaatt tgcaaaagtt ttcctccctg tattcctcac aatagttttc gtcattggac 241 ttgcaggcaa ttccatggta gtggcaattt atgcctatta caagaaacag agaaccaaaa 301 cagatgtgta catcctgaat ttggctgtag cagatttact ccttctattc actctgcctt 361 tttgggctgt taatgcagtt catgggtggg ttttagggaa aataatgtgc aaaataactt 421 cagccttgta cacactaaac tttgtctctg gaatgcagtt tctggcttgt atcagcatag 481 acagatatgt ggcagtaact aaagtcccca gccaatcagg agtgggaaaa ccatgctgga 541 tcatctgttt ctgtgtctgg atggctgcca tcttgctgag cataccccag ctggtttttt 601 atacagtaaa tgacaatgct aggtgcattc ccattttccc ccgctaccta ggaacatcaa 661 tgaaagcatt gattcaaatg ctagagatct gcattggatt tgtagtaccc tttcttatta 721 tgggggtgtg ctactttatc acagcaagga cactcatgaa gatgccaaac attaaaatat 781 ctcgacccct aaaagttctg ctcacagtcg ttatagtttt cattgtcact caactgCCTT 841 ATAACATTGT CAAGTTCTGC CGAGCCATAG ACATCATCTA CTCCCTGATC ACCAGCTGCA 901 ACATGAGCAA ACGCATGGAC ATCGCCATCC AAGTCACAGA AAGCATCGCA CTCTTTCACA 961 GCTGCCTCAA CCCAATCCTT TATGTTTTTA TGGGAGCATC TTTCAAAAAC TACGTTATGA 1021 AAGTGGCCAA GAAATATGGG TCCTGGAGAA GACAGAGACA AAGTGTGGAG GAGTTTCCTT 1081 TTGATTCTGA GGGTCCTACA GAGCCAACCA GTACTTTTAG CATTGACCCA GCTTTCTTGT 1141 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1201 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1261 GAAAGGACGA CGTCTAAGTG AGGGTAGGCG TGACACGCGT TAAGTCgaca atcaacctct 1321 ggattacaaa atttgtgaaa gatt