Construct: ORF TRCN0000487763
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021477.1_s317c1
- DNA Barcode:
- TGGCAACCAATATTGTTATAACTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ROR1 (4919)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487763
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4919 | ROR1 | receptor tyrosine kinase li... | NM_005012.4 | 99.8% | 99.8% | 180C>T;1170G>T;1553C>T |
| 2 | human | 4919 | ROR1 | receptor tyrosine kinase li... | XM_017001376.1 | 95% | 94.7% | (many diffs) |
| 3 | human | 4919 | ROR1 | receptor tyrosine kinase li... | XM_011541526.1 | 93.2% | 93.1% | 0_1ins189;981G>T;1364C>T |
| 4 | human | 4919 | ROR1 | receptor tyrosine kinase li... | XM_017001377.1 | 93.2% | 93.1% | 0_1ins189;981G>T;1364C>T |
| 5 | human | 4919 | ROR1 | receptor tyrosine kinase li... | NM_001083592.2 | 41.8% | 41.7% | (many diffs) |
| 6 | mouse | 26563 | Ror1 | receptor tyrosine kinase-li... | NM_013845.5 | 89.3% | 96.6% | (many diffs) |
| 7 | mouse | 26563 | Ror1 | receptor tyrosine kinase-li... | XM_006503121.2 | 71.4% | 78% | (many diffs) |
| 8 | mouse | 26563 | Ror1 | receptor tyrosine kinase-li... | NM_001312690.1 | 15.1% | 15.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 78
- ORF end:
- 2889
- ORF length:
- 2811
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcca attgaccatg caccggccgc gccgccgcgg gacgcgcccg ccgctcctgg 121 cgctgctggc cgcgctgctg ctggccgcac gcggggctgc tgcccaagaa acagagctgt 181 cagtcagtgc tgaattagtg cctacctcat catggaacat ctcaagtgaa ctcaacaaag 241 attcttacct gacccttgat gaaccaatga ataacatcac cacgtctctg ggccagacag 301 cagaactgca ctgcaaagtc tctgggaatc cacctcccac catccgctgg ttcaaaaatg 361 atgctcctgt ggtccaggag ccccggaggc tctcctttcg gtccaccatc tatggctctc 421 ggctgcggat tagaaacctc gacaccacag acacaggcta cttccagtgc gtggcaacaa 481 acggcaagga ggtggtttct tccactggag tcttgtttgt caagtttggc ccccctccca 541 ctgcaagtcc aggatactca gatgagtatg aagaagatgg attctgtcag ccatacagag 601 ggattgcatg tgcaagattt attggcaacc gcaccgtcta tatggagtct ttgcacatgc 661 aaggggaaat agaaaatcag atcacagctg ccttcactat gattggcact tccagtcact 721 tatctgataa gtgttctcag ttcgccattc cttccctgtg ccactatgcc ttcccgtact 781 gcgatgaaac ttcatccgtc ccaaagcccc gtgacttgtg tcgcgatgaa tgtgaaatcc 841 tggagaatgt cctgtgtcaa acagagtaca tttttgcaag atcaaatccc atgattctga 901 tgaggctgaa actgccaaac tgtgaagatc tcccccagcc agagagccca gaagctgcga 961 actgtatccg gattggaatt cccatggcag atcctataaa taaaaatcac aagtgttata 1021 acagcacagg tgtggactac cgggggaccg tcagtgtgac caaatcaggg cgccagtgcc 1081 agccatggaa ttcccagtat ccccacacac acactttcac cgcccttcgt ttcccagagc 1141 tgaatggagg ccattcctac tgccgcaacc cagggaatca aaaggaagct ccctggtgct 1201 tcaccttgga tgaaaacttt aagtctgatc tgtgtgacat cccagcttgc gattcaaagg 1261 attccaagga gaagaataaa atggaaatcc tgtacatact agtgccaagt gtggccattc 1321 ccctggccat tgctttactc ttcttcttca tttgcgtctg tcggaataac cagaagtcat 1381 cgtcggcacc agtccagagg caaccaaaac acgtcagagg tcaaaatgta gagatgtcaa 1441 tgctgaatgc atataaaccc aagagcaagg ctaaagagct acctctttct gctgtacgct 1501 ttatggaaga attgggtgag tgtgcctttg gaaaaatcta taaaggccat ctctatctcc 1561 caggcatgga ccatgctcag ctggttgcta tcaagacctt gaaagactat aacaaccccc 1621 agcaatggat ggaatttcaa caagaagcct ccctaatggc agaactgcac caccccaata 1681 ttgtctgcct tctaggtgcc gtcactcagg aacaacctgt gtgcatgctt tttgagtata 1741 ttaatcaggg ggatctccat gagttcctca tcatgagatc cccacactct gatgttggct 1801 gcagcagtga tgaagatggg actgtgaaat ccagcctgga ccacggagat tttctgcaca 1861 ttgcaattca gattgcagct ggcatggaat acctgtctag tcacttcttt gtccacaagg 1921 accttgcagc tcgcaatatt ttaatcggag agcaacttca tgtaaagatt tcagacttgg 1981 ggctttccag agaaatttac tccgctgatt actacagggt ccagagtaag tccttgctgc 2041 ccattcgctg gatgccccct gaagccatca tgtatggcaa attctcttct gattcagata 2101 tctggtcctt tggggttgtc ttgtgggaga ttttcagttt tggactccag ccatattatg 2161 gattcagtaa ccaggaagtg attgagatgg tgagaaaacg gcagctctta ccatgctctg 2221 aagactgccc acccagaatg tacagcctca tgacagagtg ctggaatgag attccttcta 2281 ggagaccaag atttaaagat attcacgtcc ggcttcggtc ctgggaggga ctctcaagtc 2341 acacaagctc tactactcct tcagggggaa atgccaccac acagacaacc tccctcagtg 2401 ccagcccagt gagtaatctc agtaacccca gatatcctaa ttacatgttc ccgagccagg 2461 gtattacacc acagggccag attgctggtt tcattggccc gccaatacct cagaaccagc 2521 gattcattcc catcaatgga tacccaatac ctcctggata tgcagcgttt ccagctgccc 2581 actaccagcc aacaggTCCT CCCAGAGTGA TTCAGCACTG CCCACCTCCC AAGAGTCGGT 2641 CCCCAAGCAG TGCCAGTGGG TCGACTAGCA CTGGCCATGT GACTAGCTTG CCCTCATCAG 2701 GATCCAATCA GGAAGCAAAT ATTCCTTTAC TACCACACAT GTCAATTCCA AATCATCCTG 2761 GTGGAATGGG TATCACCGTT TTTGGCAACA AATCTCAAAA ACCCTACAAA ATTGACTCAA 2821 AGCAAGCATC TTTACTAGGA GACGCCAATA TTCATGGACA CACCGAATCT ATGATTTCTG 2881 CAGAACTGTA AAAGCTTGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2941 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3001 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGCAAC CAATATTGTT 3061 ATAACTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt