Construct: ORF TRCN0000487810
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019475.1_s317c1
- DNA Barcode:
- TGCATCCGGATCGTACGTGCTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR1D (3352)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487810
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3352 | HTR1D | 5-hydroxytryptamine recepto... | NM_000864.4 | 99.8% | 99.7% | 585C>T;1131_1132insG |
| 2 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_001285482.1 | 84.9% | 89.9% | (many diffs) |
| 3 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_001285483.1 | 84.9% | 89.9% | (many diffs) |
| 4 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_001285484.1 | 84.9% | 89.9% | (many diffs) |
| 5 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | NM_008309.5 | 84.9% | 89.9% | (many diffs) |
| 6 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | XM_006538577.3 | 84.9% | 89.9% | (many diffs) |
| 7 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | XM_006538578.2 | 84.9% | 89.9% | (many diffs) |
| 8 | mouse | 15552 | Htr1d | 5-hydroxytryptamine (seroto... | XM_017319986.2 | 84.9% | 89.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1209
- ORF length:
- 1134
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtcc ccactgaacc agtcagcaga aggccttccc caggaggcct 121 ccaacagatc cctgaatgcc acagaaacct cagaggcttg ggatcccagg accctccagg 181 cgctcaagat ctcccttgcc gtggtccttt ccgtcatcac actggccaca gtcctctcca 241 atgcctttgt actcaccacc atcttactca ccaggaagct ccacacccct gccaactacc 301 tgattggctc cctggccacc accgacctct tggtttccat cttggtaatg cccatcagca 361 tcgcctatac catcacccac acctggaact ttggccaaat cttgtgtgac atctggctgt 421 cctctgacat cacgtgctgc acagcctcca tcctgcatct ctgtgtcatt gctctggaca 481 ggtactgggc aatcacagat gccctggaat acagtaaacg caggacggct ggccacgcgg 541 ccaccatgat cgccattgtc tgggccatct ccatctgcat ctccatcccc ccgctcttct 601 ggcggcaggc caaggcccag gaggagatgt cggactgtct ggtgaacacc tctcagattt 661 cctacaccat ctactccacc tgtggggcct tctacattcc ctcggtgttg ctcatcatcc 721 tatatggccg gatctaccgg gctgcccgga accgcatcct gaatccaccc tcactctatg 781 ggaagcgctt caccacggcc cacctcatca caggctctgc cgggtcctcg ctctgctcgc 841 tcaactccag cctccatgag gggcactcgc actcggctgg ctcccctctc tttttcaacc 901 acgtgaaaat caagcttgct gacagtgccc TGGAACGCAA GAGGATTTCT GCTGCTCGAG 961 AAAGGAAAGC CACTAAAATC CTGGGCATCA TTCTGGGGGC CTTTATCATC TGCTGGCTGC 1021 CCTTCTTCGT GGTGTCTCTG GTCCTCCCCA TCTGCCGGGA CTCCTGCTGG ATCCACCCGG 1081 CGCTCTTTGA CTTCTTCACC TGGCTAGGCT ATTTAAACTC CCTCATCAAT CCAATAATCT 1141 ACACTGTGTT TAATGAAGAG TTTCGGCAAG CTTTTCAGAA AATTGTCCCT TTCCGGAAGG 1201 CCTCCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGCATCCGG ATCGTACGTG CTCTCACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt