Construct: ORF TRCN0000487817

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020605.1_s317c1
DNA Barcode:
GGACCCAGTCAATTGCACAATCGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MARK2 (2011)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487817
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2011 MARK2 microtubule affinity regula... NM_004954.5 100% 100%
2 human 2011 MARK2 microtubule affinity regula... NM_001163296.2 98.5% 98.2% (many diffs)
3 human 2011 MARK2 microtubule affinity regula... NM_001163297.2 97.9% 97.9% 1766_1767ins45
4 human 2011 MARK2 microtubule affinity regula... XM_011544796.1 96.3% 95.6% (many diffs)
5 human 2011 MARK2 microtubule affinity regula... XM_011544795.1 96.2% 95.5% (many diffs)
6 human 2011 MARK2 microtubule affinity regula... XM_011544794.1 95.1% 94.5% (many diffs)
7 human 2011 MARK2 microtubule affinity regula... XM_017017311.1 95% 94% (many diffs)
8 human 2011 MARK2 microtubule affinity regula... XM_011544797.1 94.9% 93.9% (many diffs)
9 human 2011 MARK2 microtubule affinity regula... XM_011544798.1 94.3% 93.6% (many diffs)
10 human 2011 MARK2 microtubule affinity regula... NM_001039469.2 91.8% 91.8% 1234_1236delGCA;1512_1673del;1933_1959del
11 human 2011 MARK2 microtubule affinity regula... XM_017017310.1 91.1% 91.1% 1509_1670del;1928_1929ins45
12 human 2011 MARK2 microtubule affinity regula... XM_011544791.1 89.8% 89.2% (many diffs)
13 human 2011 MARK2 microtubule affinity regula... XM_011544790.1 89.7% 89.1% (many diffs)
14 human 2011 MARK2 microtubule affinity regula... NM_017490.4 88.8% 88.8% 0_1ins99;1410_1571del
15 human 2011 MARK2 microtubule affinity regula... XM_011544789.1 88.7% 88.1% (many diffs)
16 human 2011 MARK2 microtubule affinity regula... XM_011544792.1 88.5% 87.6% (many diffs)
17 human 2011 MARK2 microtubule affinity regula... XM_011544793.1 87.8% 87.2% (many diffs)
18 human 2011 MARK2 microtubule affinity regula... XR_949801.2 80.6% (many diffs)
19 human 2011 MARK2 microtubule affinity regula... XR_949802.2 71.5% 1_552del;2725_3035del
20 human 2011 MARK2 microtubule affinity regula... XR_002957128.1 47% (many diffs)
21 mouse 13728 Mark2 MAP/microtubule affinity re... NM_001080388.2 92.5% 97.9% (many diffs)
22 mouse 13728 Mark2 MAP/microtubule affinity re... NM_001080389.2 91.4% 96.7% (many diffs)
23 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526652.3 89.1% 92.8% (many diffs)
24 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526654.3 88.9% 92.4% (many diffs)
25 mouse 13728 Mark2 MAP/microtubule affinity re... XM_017318058.1 88.2% 91.9% (many diffs)
26 mouse 13728 Mark2 MAP/microtubule affinity re... XM_017318059.1 88.1% 93.3% (many diffs)
27 mouse 13728 Mark2 MAP/microtubule affinity re... NM_007928.3 86.1% 91.1% (many diffs)
28 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526648.3 83.1% 86.6% (many diffs)
29 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526647.2 82.9% 86.4% (many diffs)
30 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526650.3 82.8% 86% (many diffs)
31 mouse 13728 Mark2 MAP/microtubule affinity re... NM_001080390.2 82% 86.8% (many diffs)
32 mouse 13728 Mark2 MAP/microtubule affinity re... XM_011247139.1 80% 84.9% (many diffs)
33 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526643.2 79.5% 84.1% (many diffs)
34 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526642.3 78.5% 81.8% (many diffs)
35 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526641.3 77.7% 80.9% (many diffs)
36 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526644.3 75.6% 80.1% (many diffs)
37 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526645.2 75.6% 80.1% (many diffs)
38 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526646.3 75.6% 80.1% (many diffs)
39 mouse 13728 Mark2 MAP/microtubule affinity re... XM_006526656.3 72.3% 72.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2244
ORF length:
2172
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtccagc gctcggaccc ccctacccac gctgaacgag agggacacgg
121agcagcccac cttgggacac cttgactcca agcccagcag taagtccaac atgattcggg
181gccgcaactc agccacctct gctgatgagc agccccacat tggaaactac cggctcctca
241agaccattgg caagggtaat tttgccaagg tgaagttggc ccgacacatc ctgactggga
301aagaggtagc tgtgaagatc attgacaaga ctcaactgaa ctcctccagc ctccagaaac
361tattccgcga agtaagaata atgaaggttt tgaatcatcc caacatagtt aaattatttg
421aagtgattga gactgagaaa acgctctacc ttgtcatgga gtacgctagt ggcggagagg
481tatttgatta cctagtggct catggcagga tgaaagaaaa agaggctcga gccaaattcc
541gccagatagt gtctgctgtg cagtactgtc accagaagtt tattgtccat agagacttaa
601aggcagaaaa cctgctcttg gatgctgata tgaacatcaa gattgcagac tttggcttca
661gcaatgaatt cacctttggg aacaagctgg acaccttctg tggcagtccc ccttatgctg
721ccccagaact cttccagggc aaaaaatatg atggacccga ggtggatgtg tggagcctag
781gagttatcct ctatacactg gtcagcggat ccctgccttt tgatggacag aacctcaagg
841agctgcggga acgggtactg aggggaaaat accgtattcc attctacatg tccacggact
901gtgaaaacct gcttaagaaa tttctcattc ttaatcccag caagagaggc actttagagc
961aaatcatgaa agatcgatgg atgaatgtgg gtcacgaaga tgatgaacta aagccttacg
1021tggagccact ccctgactac aaggaccccc ggcggacaga gctgatggtg tccatgggtt
1081atacacggga agagatccag gactcgctgg tgggccagag atacaacgag gtgatggcca
1141cctatctgct cctgggctac aagagctccg agctggaagg cgacaccatc accctgaaac
1201cccggccttc agctgatctg accaatagca gcgccccatc cccatcccac aaggtacagc
1261gcagcgtgtc ggccaatccc aagcagcggc gcttcagcga ccaggctggt cctgccattc
1321ccacctctaa ttcttactct aagaagactc agagtaacaa cgcagaaaat aagcggcctg
1381aggaggaccg ggagtcaggg cggaaagcca gcagcacagc caaggtgcct gccagccccc
1441tgcccggtct ggagaggaag aagaccaccc caaccccctc cacgaacagc gtcctctcca
1501ccagcacaaa tcgaagcagg aattccccac ttttggagcg ggccagcctc ggccaggcct
1561ccatccagaa tggcaaagac agcacagccc cccagcgtgt ccctgttgcc tccccatccg
1621cccacaacat cagcagcagt ggtggagccc cagaccgaac taacttcccc cggggtgtgt
1681ccagccgaag caccttccat gctgggcagc tccgacaggt gcgggaccag cagaatttgc
1741cctacggtgt gaccccagcc tctccctctg gccacagcca gggccggcgg ggggcctctg
1801ggagcatctt cagcaagttc acctccaagt ttgtacgcag gaacctgaat gaacctgaaa
1861gcaaagaccg agtggagacg ctcagacctc acgtggtggg cagtggcggc aacgacaaag
1921aaaaggaaga atttcgggag gccaagcccc gctcccTCCG CTTCACGTGG AGTATGAAGA
1981CCACGAGCTC CATGGAGCCC AACGAGATGA TGCGGGAGAT CCGCAAGGTG CTGGACGCGA
2041ACAGCTGCCA GAGCGAGCTG CATGAGAAGT ACATGCTGCT GTGCATGCAC GGCACGCCGG
2101GCCACGAGGA CTTCGTGCAG TGGGAGATGG AGGTGTGCAA ACTGCCGCGG CTCTCTCTCA
2161ACGGGGTTCG ATTTAAGCGG ATATCGGGCA CCTCCATGGC CTTCAAAAAC ATTGCCTCCA
2221AAATAGCCAA CGAGCTGAAG CTTTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
2281GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2341AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGACCCAG
2401TCAATTGCAC AATCGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2461aagatt

Download FASTA (ORF) (Full)

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