Construct: ORF TRCN0000487824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021249.1_s317c1
- DNA Barcode:
- TATACGAGGTAGACCTATGCCGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LYZL4 (131375)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487824
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 131375 | LYZL4 | lysozyme like 4 | NM_001304386.2 | 100% | 100% | |
| 2 | human | 131375 | LYZL4 | lysozyme like 4 | NM_144634.4 | 100% | 100% | |
| 3 | human | 131375 | LYZL4 | lysozyme like 4 | XM_017005706.1 | 100% | 100% | |
| 4 | human | 131375 | LYZL4 | lysozyme like 4 | XM_024453345.1 | 100% | 100% | |
| 5 | human | 131375 | LYZL4 | lysozyme like 4 | XM_011533355.3 | 88% | 83.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 510
- ORF length:
- 438
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaggca tccgtggttc tctccctcct tggctacctg gtggttccaa 121 gtggtgctta catcttgggg cgttgcacag tggctaagaa actccacgat ggaggcctgg 181 attattttga gggctatagc cttgagaact gggtgtgcct ggccTACTTC GAGAGCAAGT 241 TCAACCCCAT GGCCATCTAC GAGAACACAC GTGAGGGCTA CACTGGCTTT GGCCTCTTTC 301 AGATGCGTGG CAGTGACTGG TGTGGCGACC ATGGCAGGAA CCGCTGCCAT ATGTCATGTT 361 CCGCTTTACT GAATCCTAAT TTAGAGAAGA CAATTAAATG TGCCAAGACC ATTGTAAAAG 421 GAAAAGAAGG GATGGGAGCA TGGCCCACCT GGTCCCGGTA CTGCCAGTAC TCCGATACCC 481 TGGCACGGTG GCTGGATGGT TGCAAGCTGT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 661 TACGAGGTAG ACCTATGCCG ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt