Construct: ORF TRCN0000487838
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021165.1_s317c1
- DNA Barcode:
- GAACCAGCCGAACGTAAACCGATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRE2 (30817)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487838
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | NM_013447.4 | 99.6% | 99.5% | (many diffs) |
| 2 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XM_011527948.2 | 97.8% | 97.6% | (many diffs) |
| 3 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XM_011527949.2 | 96.5% | 96.2% | (many diffs) |
| 4 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XM_017026726.1 | 93.1% | 92.9% | (many diffs) |
| 5 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | NM_001271052.1 | 92.6% | 92.4% | (many diffs) |
| 6 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XM_011527951.3 | 92% | 91.7% | (many diffs) |
| 7 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XM_011527952.3 | 90.9% | 90.6% | (many diffs) |
| 8 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XM_011527953.2 | 87% | 86.8% | (many diffs) |
| 9 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XM_011527954.3 | 83.7% | 83.5% | (many diffs) |
| 10 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XM_017026727.1 | 83.7% | 83.5% | (many diffs) |
| 11 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XM_011527955.2 | 73.2% | 73.1% | (many diffs) |
| 12 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XR_001753674.1 | 36.7% | (many diffs) | |
| 13 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XR_001753675.1 | 34.3% | (many diffs) | |
| 14 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XR_936173.1 | 33.8% | (many diffs) | |
| 15 | human | 30817 | ADGRE2 | adhesion G protein-coupled ... | XR_936174.1 | 31.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2541
- ORF length:
- 2469
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggaggc cgcgtctttc tcgtctttct cgcattctgt gtctggctga 121 ctctgccggg agctgaaacc caggactcca ggggctgtgc ccggtggtgc cctcaggact 181 cctcgtgtgt caatgccacc gcctgtcgct gcaatccagg gttcagctct ttttctgaga 241 tcatcaccac ccccatggag acttgtgacg acatcaacga gtgtgcaaca ctgtcgaaag 301 tgtcatgcgg aaaattctcg gactgctgga acacagaggg gagctacgac tgcgtgtgca 361 gcccaggata tgagcctgtt tctggggcaa aaacattcaa gaatgagagc gagaacacgt 421 gtcaagatgt ggacgaatgt cagcagaacc caaggctctg taaaagctac ggcacctgcg 481 tcaacaccct tggcagctat acctgccagt gcctgcctgg cttcaagttc atacctgagg 541 atccgaaggt ctgcacagat gtgaatgaat gcacctccgg acaaaaccca tgccacagct 601 ccacccactg cctcaacaac gtgggcagct atcagtgccg ctgccgcccg ggctggcaac 661 cgattccggg gtcccccaat ggcccaaaca ataccgtctg tgaagatgtg gacgagtgca 721 gctccgggca gcatcagtgt gacagctcca ccgtctgctt caacaccgtg ggttcataca 781 gctgccgctg ccgcccaggc tggaagccca gacacggaat cccgaataac caaaaggaca 841 ctgtctgtga agatatgact ttctccacct ggaccccgcc ccctggagtc cacagccaga 901 cgctttcccg attcttcgac aaagtccagg acctgggcag agactacaag ccaggcttgg 961 ccaataacac catccagagc atcttacagg cgctggatga gctgctggag gtccctgggg 1021 acctggagac cctgccccgc ttacagcagc actgtgtggc cagtcacctg ctggatggcc 1081 tagaggatgt cctcagaggc ctgagcaaga acctttccaa tgggctgttg aacttcagtt 1141 atcctgcagg cacagaattg tccctggagg tgcagaagca agtagacagg agtgtcacct 1201 tgagacagaa tcaggcagtg atgcagctcg actggaatca ggcacagaaa tctggtgacc 1261 caggcccttc tgtggtgggc cttgtctcca ttccagggat gggcaagttg ctggctgagg 1321 cccctctggt cctggaacct gagaagcaga tgcttctgca tgagacacac cagggcttgc 1381 tgcaggacgg ctcccccatc ctgctctcag atgtgatctc tgcctttctg agcaacaacg 1441 acacccaaaa cctcagctcc ccagttacct tcaccttctc ccaccgttca gtgatcccga 1501 gacagaaggt gctctgtgtc ttctgggagc atggccagaa tggatgtggt cactgggcca 1561 ccacaggctg cagcacaata ggcaccagag acaccagcac catctgccgt tgcacccacc 1621 tgagcagctt tgccgtcctc atggcccact acgatgtgca ggaggaggat cccgtgctga 1681 ctgtcatcac ctacatgggg ctgagcgtct ctctgctgtg cctcctcctg gcggccctca 1741 cttttctcct gtgtaaagcc atccagaaca ccagcacctc actgcatctg cagctctcgc 1801 tctgcctctt cctggcccac ctcctcttcc tcgtggcaat tgatcaaacc ggacacaagg 1861 tgctgtgctc catcatcgcc ggtaccttgc actatctcta cctggccacc ttgacctgga 1921 tgctgctgga ggccctgtac ctcttcctca ctgcacggaa cctgacggtg gtcaactact 1981 caagcatcaa cagattcatg aagaagctca tgttccctgt gggctacgga gtcccagctg 2041 tgacagtggc catttctgca gcctccaggc ctcaccttta tggaacacct tcccgctgct 2101 ggctccaacc agaaaaggga tttatatggg gcttccttgg acctgtctgc gccatcttct 2161 ctgtgaattt agttctcttt ctggtgactc tctggatttt gaaaaacaga ctctcctccc 2221 tcaatagtga agtgtccacc cTCCGGAACA CAAGGATGCT GGCATTTAAA GCGACAGCTC 2281 AGCTGTTCAT CCTGGGCTGC ACGTGGTGTC TGGGCATCTT GCAGGTGGGT CCGGCTGCCC 2341 GGGTCATGGC CTACCTCTTC ACCATCATCA ACAGCCTGCA GGGTGTCTTC ATCTTCCTGG 2401 TGTACTGCCT CCTCAGCCAG CAGGTCCGGG AGCAATATGG GAAATGGTCC AAAGGGATCA 2461 GGAAATTGAA AACTGAGTCT GAGATGCACA CACTCTCCAG CAGTGCTAAG GCTGACACCT 2521 CCAAACCCAG CACGGTTAAC TAGGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2581 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2641 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG AACCAGCCGA 2701 ACGTAAACCG ATGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2761 att