Construct: ORF TRCN0000487839

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020702.1_s317c1
DNA Barcode:
TTGGACACTTTAGTTCTCCCGCAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CLK2 (1196)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487839
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1196 CLK2 CDC like kinase 2 NM_003993.4 100% 100%
2 human 1196 CLK2 CDC like kinase 2 NM_001294338.2 99.7% 99.7% 399_401delGCA
3 human 1196 CLK2 CDC like kinase 2 NM_001363704.2 99.7% 99.7% 168_169insAGC
4 human 1196 CLK2 CDC like kinase 2 XM_011509143.2 99.5% 99.5% 168_169insAGC;396_398delGCA
5 human 1197 CLK2P1 CDC like kinase 2, pseudoge... NR_002711.3 61.6% (many diffs)
6 human 1196 CLK2 CDC like kinase 2 NM_001294339.2 54.4% 54.4% 0_1ins681
7 mouse 12748 Clk2 CDC-like kinase 2 NM_007712.4 92.3% 96.7% (many diffs)
8 mouse 12748 Clk2 CDC-like kinase 2 NM_001163432.2 92.2% 96.5% (many diffs)
9 mouse 105943584 Gm45927 predicted gene, 45927 NM_001310016.1 56.9% 57.1% (many diffs)
10 mouse 105943584 Gm45927 predicted gene, 45927 NM_001310017.1 30.6% 28.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1566
ORF length:
1494
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgccgcat cctcgaaggt accactcctc agagcgaggc agccggggga
121gttaccgtga acactatcgg agccgaaagc ataagcgacg aagaagtcgc tcctggtcaa
181gtagtagtga ccggacacga cggcgtcggc gagaggacag ctaccatgtc cgttctcgaa
241gcagttatga tgatcgttcg tccgaccgga gggtgtatga ccggcgatac tgtggcagct
301acagacgcaa cgattatagc cgggatcggg gagatgccta ctatgacaca gactatcggc
361attcctatga atatcagcgg gagaacagca gttaccgcag ccagcgcagc agccggagga
421agcacagacg gcggaggagg cgcagccgga catttagccg ctcatcttcg cacagcagcc
481ggagagccaa gagtgtagag gacgacgctg agggccacct catctaccac gtcggggact
541ggctacaaga gcgatatgaa atcgttagca ccttaggaga ggggaccttc ggccgagttg
601tacaatgtgt tgaccatcgc aggggtgggg ctcgagttgc cctgaagatc attaagaatg
661tggagaagta caaggaagca gctcgacttg agatcaacgt gctagagaaa atcaatgaga
721aagaccctga caacaagaac ctctgtgtcc agatgtttga ctggtttgac taccatggcc
781acatgtgtat ctcctttgag cttctgggcc ttagcacctt cgatttcctc aaagacaaca
841actacctgcc ctaccccatc caccaagtgc gccacatggc cttccagctg tgccaggctg
901tcaagttcct ccatgataac aagctgacac atacagacct caagcctgaa aatattctgt
961ttgtgaattc agactatgag ctcacctaca acctagagaa gaagcgagat gagcgcagtg
1021tgaagagcac agctgtgcgg gtggtagact ttggcagtgc cacctttgac catgagcacc
1081atagcaccat tgtctccact cgccattacc gagcaccaga agtcatcctt gagttgggct
1141ggtcacagcc ttgtgatgtg tggagtatag gctgcatcat ctttgaatac tatgtgggat
1201tcaccctctt ccagacccat gacaacagag agcatctagc catgatggaa aggatcttgg
1261gtcctatccc TTCCCGGATG ATCCGAAAGA CAAGAAAGCA GAAATATTTT TACCGGGGTC
1321GCCTGGATTG GGATGAGAAC ACATCAGCTG GGCGCTATGT TCGTGAGAAC TGCAAACCGC
1381TGCGGCGGTA TCTGACCTCA GAGGCAGAGG AACACCACCA GCTCTTCGAT CTGATTGAAA
1441GCATGCTAGA GTATGAACCA GCTAAGCGGC TGACCTTGGG TGAAGCCCTT CAGCATCCTT
1501TCTTCGCCCG CCTTCGGGCT GAGCCGCCCA ACAAGTTGTG GGACTCCAGT CGGGATATCA
1561GTCGGTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1621CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1681TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTGGAC ACTTTAGTTC TCCCGCAAAC
1741GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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