Construct: ORF TRCN0000487843
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021896.1_s317c1
- DNA Barcode:
- CTGCCGGGATGTCGATATGCACCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CERT1 (10087)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487843
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10087 | CERT1 | ceramide transporter 1 | NM_031361.3 | 100% | 100% | |
| 2 | human | 10087 | CERT1 | ceramide transporter 1 | XM_017008919.2 | 100% | 100% | |
| 3 | human | 10087 | CERT1 | ceramide transporter 1 | NM_005713.3 | 95.8% | 95.8% | 1109_1186del |
| 4 | human | 10087 | CERT1 | ceramide transporter 1 | XM_006714513.3 | 95.8% | 95.8% | 1109_1186del |
| 5 | human | 10087 | CERT1 | ceramide transporter 1 | XM_011543091.3 | 94.4% | 90.6% | (many diffs) |
| 6 | human | 10087 | CERT1 | ceramide transporter 1 | XM_011543090.3 | 90.6% | 87% | (many diffs) |
| 7 | human | 10087 | CERT1 | ceramide transporter 1 | NM_001130105.1 | 79.5% | 79.5% | 1_384del;1493_1570del |
| 8 | mouse | 68018 | Col4a3bp | collagen, type IV, alpha 3 ... | NM_001164222.1 | 90.2% | 96.4% | (many diffs) |
| 9 | mouse | 68018 | Col4a3bp | collagen, type IV, alpha 3 ... | NM_023420.2 | 86.4% | 92.4% | (many diffs) |
| 10 | mouse | 68018 | Col4a3bp | collagen, type IV, alpha 3 ... | XM_011244694.2 | 66.7% | 71.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1866
- ORF length:
- 1794
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcggat aatcagagct ggaactcgtc gggctcggag gaggatccag 121 agacggagtc tgggccgcct gtggagcgct gcggggtcct cagtaagtgg acaaactaca 181 ttcatgggtg gcaggatcgt tgggtagttt tgaaaaataa tgctctgagt tactacaaat 241 ctgaagatga aacagagtat ggctgcagag gatccatctg tcttagcaag gctgtcatca 301 cacctcacga ttttgatgaa tgtcgatttg atattagtgt aaatgatagt gtttggtatc 361 ttcgtgctca ggatccagat catagacagc aatggataga tgccattgaa cagcacaaga 421 ctgaatctgg atatggatct gaatccagct tgcgtcgaca tggctcaatg gtgtccctgg 481 tgtctggagc aagtggctac tctgcaacat ccacctcttc attcaagaaa ggccacagtt 541 tacgtgagaa gttggctgaa atggaaacat ttagagacat cttatgtaga caagttgaca 601 cgctacagaa gtactttgat gcctgtgctg atgctgtctc taaggatgaa cttcaaaggg 661 ataaagtggt agaagatgat gaagatgact ttcctacaac gcgttctgat ggtgacttct 721 tgcatagtac caacggcaat aaagaaaagt tatttccaca tgtgacacca aaaggaatta 781 atggtataga ctttaaaggg gaagcgataa cttttaaagc aactactgct ggaatccttg 841 caacactttc tcattgtatt gaactaatgg ttaaacgtga ggacagctgg cagaagagac 901 tggataagga aactgagaag aaaagaagaa cagaggaagc atataaaaat gcaatgacag 961 aacttaagaa aaaatcccac tttggaggac cagattatga agaaggccct aacagtctga 1021 ttaatgaaga agagttcttt gatgctgttg aagctgctct tgacagacaa gataaaatag 1081 aagaacagtc acagagtgaa aaggtgagat tacattggcc tacatccttg ccctctggag 1141 atgccttttc ttctgtgggg acacatagat ttgtccaaaa ggttgaagag atggtgcaga 1201 accacatgac ttactcatta caggatgtag gcggagatgc caattggcag ttggttgtag 1261 aagaaggaga aatgaaggta tacagaagag aagtagaaga aaatgggatt gttctggatc 1321 ctttaaaagc tacccatgca gttaaaggcg tcacaggaca tgaagtctgc aattatttct 1381 ggaatgttga cgttcgcaat gactgggaaa caactataga aaactttcat gtggtggaaa 1441 cattagctga taatgcaatc atcatttatc aaacacacaa gagggtgtgg cctgcttctc 1501 agcgagacgt attatatctt tctgtcattc gaaagatacc agccttgact gaaaatgacc 1561 ctgaaacttG GATAGTTTGT AATTTTTCTG TGGATCATGA CAGTGCTCCT CTAAACAACC 1621 GATGTGTCCG TGCCAAAATA AATGTTGCTA TGATTTGTCA AACCTTGGTA AGCCCACCAG 1681 AGGGAAACCA GGAAATTAGC AGGGACAACA TTCTATGCAA GATTACATAT GTAGCTAATG 1741 TGAACCCTGG AGGATGGGCA CCAGCCTCAG TGTTAAGGGC AGTGGCAAAG CGAGAGTATC 1801 CTAAATTTCT AAAACGTTTT ACTTCTTACG TCCAAGAAAA AACTGCAGGA AAGCCTATTT 1861 TGTTCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1921 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1981 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTGCCG GGATGTCGAT ATGCACCTAC 2041 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt