Construct: ORF TRCN0000487849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021993.1_s317c1
- DNA Barcode:
- TAAATCGAGAGATATGCACCCAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLCO1B3 (28234)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487849
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28234 | SLCO1B3 | solute carrier organic anio... | NM_019844.4 | 99.8% | 99.7% | 334T>G;699G>A;1557A>G |
| 2 | human | 28234 | SLCO1B3 | solute carrier organic anio... | NM_001349920.2 | 95.8% | 95.7% | (many diffs) |
| 3 | human | 115072896 | SLCO1B3-SLCO1B7 | SLCO1B3-SLCO1B7 readthrough | NM_001371097.1 | 88.5% | 86.7% | (many diffs) |
| 4 | human | 10599 | SLCO1B1 | solute carrier organic anio... | NM_006446.5 | 85.7% | 78.5% | (many diffs) |
| 5 | human | 338821 | SLCO1B7 | solute carrier organic anio... | NM_001009562.4 | 79.9% | 71% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2175
- ORF length:
- 2106
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaccaacat caacatttga ataaaacagc agagtcagca tcttcagaga 121 aaaagaaaac aagacgctgc aatggattca agatgttctt ggcagccctg tcattcagct 181 atattgctaa agcactaggt ggaatcatta tgaaaatttc catcactcaa atagaaagga 241 gatttgacat atcctcttct cttgctggtt taattgatgg aagctttgaa attggaaatt 301 tgcttgtgat tgtatttgta agttactttg gatctaaact acacagaccg aagttaattg 361 gaattggttg tctccttatg ggaactggaa gtattttgac agctttacca catttcttca 421 tgggatatta taggtattct aaagaaaccc atattaatcc atcagaaaat tcaacatcaa 481 gtttatcaac ctgtttaatt aatcaaacct tatcattcaa tggaacatca cctgagatag 541 tagaaaaaga ttgtgtaaag gaatctgggt cacacatgtg gatctatgtc ttcatgggga 601 atatgcttcg tggcataggg gaaaccccca tagtaccatt ggggatttca tacattgatg 661 attttgcaaa agaaggacat tcttccttgt atttaggtag tttgaatgca ataggaatga 721 ttggtccagt cattggcttt gcactgggat ctctgtttgc taaaatatac gtggatattg 781 gatatgtaga tctgagcact atcagaataa ctcctaagga ctctcgttgg gttggagctt 841 ggtggcttgg tttccttgtg tctggactat tttccattat ttcttccata ccattttttt 901 tcttgccgaa aaatccaaat aaaccacaaa aagaaagaaa aatttcacta tcattgcatg 961 tgctgaaaac aaatgatgat agaaatcaaa cagctaattt gaccaaccaa ggaaaaaatg 1021 ttaccaaaaa tgtgactggt tttttccagt ctttgaaaag catccttacc aatcccctgt 1081 atgttatatt tctgcttttg acattgttac aagtaagcag ctttattggt tcttttactt 1141 acgtctttaa atatatggag caacagtacg gtcagtctgc atctcatgct aactttttgt 1201 tgggaatcat aaccattcct acggttgcaa ctggaatgtt tttaggagga tttatcatta 1261 aaaaattcaa attgtcttta gttggaattg ccaaattttc atttcttact tcgatgatat 1321 ccttcttgtt tcaacttcta tatttccctc taatctgcga aagcaaatca gttgccggcc 1381 taaccttgac ctatgatgga aataattcag tggcatctca tgtagatgta ccactttctt 1441 attgcaactc agagtgcaat tgtgatgaaa gtcagtggga accagtctgt gggaacaatg 1501 gaataactta cctgtcacct tgtctagcag gatgcaaatc ctcaagtggt attaaaaagc 1561 atacagtgtt ttataactgt agttgtgtgg aagtaactgg tctccagaac agaaattact 1621 cagcgcactt gggtgaatgc ccaagagata atacttgtac aaggaaattt ttcatctatg 1681 ttgcaattca agtcataaac tctttgttct ctgcaacagg aggtaccaca tttatcttgt 1741 tgactgtgaa gattgttcaa cctgaattga aagcacttgc aatgggtttc cagtcaatgg 1801 ttataagaac actaggagga attctagctc caatatattt tggggctctg attgataaaa 1861 catgtatgaa gtggtccacc aacagctgtg gagcacaagg ggcttgtagg atatataatt 1921 ccgtattttt tGGAAGGGTC TACTTGGGCT TATCTATAGC TTTAAGATTC CCAGCACTTG 1981 TTTTATATAT TGTTTTCATT TTTGCTATGA AGAAAAAATT TCAAGGAAAA GATACCAAGG 2041 CATCGGACAA TGAAAGAAAA GTAATGGATG AAGCAAACTT AGAATTCTTA AATAATGGTG 2101 AACATTTTGT ACCTTCTGCT GGAACAGATA GTAAAACATG TAATTTGGAC ATGCAAGACA 2161 ATGCTGCTGC CAACTAAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2221 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2281 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATAAATCG AGAGATATGC 2341 ACCCAACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt