Construct: ORF TRCN0000487853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020563.1_s317c1
- DNA Barcode:
- CAACTATGGACGGGAACTAGTAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SRPK1 (6732)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6732 | SRPK1 | SRSF protein kinase 1 | NM_003137.5 | 100% | 100% | |
| 2 | human | 6732 | SRPK1 | SRSF protein kinase 1 | NR_034069.1 | 44.5% | 1_124del;199_226del;2118_4408del | |
| 3 | mouse | 20815 | Srpk1 | serine/arginine-rich protei... | NM_016795.4 | 88.3% | 91.6% | (many diffs) |
| 4 | mouse | 20815 | Srpk1 | serine/arginine-rich protei... | XM_006523958.3 | 76.7% | 78.3% | (many diffs) |
| 5 | mouse | 20815 | Srpk1 | serine/arginine-rich protei... | XM_011246337.2 | 74.8% | 75.5% | (many diffs) |
| 6 | mouse | 20815 | Srpk1 | serine/arginine-rich protei... | XR_385308.3 | 57.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2037
- ORF length:
- 1965
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagcgg aaagtgcttg cgctccaggc ccgaaagaaa aggaccaagg 121 ccaagaagga caaagcccaa aggaaatctg aaactcagca ccgaggctct gctccccact 181 ctgagagtga tctaccagag caggaagagg agattctggg atctgatgat gatgagcaag 241 aagatcctaa tgattattgt aaaggaggtt atcatcttgt gaaaattgga gatctattca 301 atgggagata ccatgtgatc cgaaagttag gctggggaca cttttcaaca gtatggttat 361 catgggatat tcaggggaag aaatttgtgg caatgaaagt agttaaaagt gctgaacatt 421 acactgaaac agcactagat gaaatccggt tgctgaagtc agttcgcaat tcagacccta 481 atgatccaaa tagagaaatg gttgttcaac tactagatga ctttaaaata tcaggagtta 541 atggaacaca tatctgcatg gtatttgaag ttttggggca tcatctgctc aagtggatca 601 tcaaatccaa ttatcagggg cttccactgc cttgtgtcaa aaaaattatt cagcaagtgt 661 tacagggtct tgattattta cataccaagt gccgtatcat ccacactgac attaaaccag 721 agaacatctt attgtcagtg aatgagcagt acattcggag gctggctgca gaagcaacag 781 aatggcagcg atctggagct cctccgcctt ccggatctgc agtcagtact gctccccagc 841 ctaaaccagc tgacaaaatg tcaaagaata agaagaagaa attgaagaag aagcagaagc 901 gccaggcaga attactagag aagcgaatgc aggaaattga ggaaatggag aaagagtcgg 961 gccctgggca aaaaagacca aacaagcaag aagaatcaga gagtcctgtt gaaagaccct 1021 tgaaagagaa cccacctaat aaaatgaccc aagaaaaact tgaagagtca agtaccattg 1081 gccaggatca aacgcttatg gaacgtgata cagagggtgg tgcagcagaa attaattgca 1141 atggagtgat tgaagtcatt aattatactc agaacagtaa taatgaaaca ttgagacata 1201 aagaggatct acataatgct aatgactgtg atgtccaaaa tttgaatcag gaatctagtt 1261 tcctaagctc ccaaaatgga gacagcagca catctcaaga aacagactct tgtacaccta 1321 taacatctga ggtgtcagac accatggtgt gccagtcttc ctcaactgta ggtcagtcat 1381 tcagtgaaca acacattagc caacttcaag aaagcattcg ggcagagata ccctgtgaag 1441 atgaacaaga gcaagaacat aacggaccac tggacaacaa aggaaaatcc acggctggaa 1501 attttcttgt taatcccctt gagccaaaaa atgcagaaaa gctcaaggtg aagattgctg 1561 accttggaaa tgcttgttgg gtgcacaaac atttcactga agatattcaa acaaggcaat 1621 atcgttcctt ggaagttcta atcggatctg gctataatac ccctgctgac atttggagca 1681 cggcatgcat ggcctttgaa ctggccacag gtgactattt gtttgaacct cattcagggG 1741 AAGAGTACAC TCGAGATGAA GATCACATTG CATTGATCAT AGAACTTCTG GGGAAGGTGC 1801 CTCGCAAGCT CATTGTGGCA GGAAAATATT CCAAGGAATT TTTCACCAAA AAAGGTGACC 1861 TGAAACATAT CACGAAGCTG AAACCTTGGG GCCTTTTTGA GGTTCTAGTG GAGAAGTATG 1921 AGTGGTCGCA GGAAGAGGCA GCTGGCTTCA CAGATTTCTT ACTGCCCATG TTGGAGCTGA 1981 TCCCTGAGAA GAGAGCCACT GCCGCCGAGT GTCTCCGGCA CCCTTGGCTT AACTCCTAAA 2041 AGCTTGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2101 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2161 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAACTATGG ACGGGAACTA GTAACACGCG 2221 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt