Construct: ORF TRCN0000487866
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021419.1_s317c1
- DNA Barcode:
- GTATATGCGCCTCCTCATTGTCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OLFML2B (25903)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487866
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25903 | OLFML2B | olfactomedin like 2B | XM_011509398.2 | 80.1% | 80% | 1_303del;688T>C |
| 2 | human | 25903 | OLFML2B | olfactomedin like 2B | NM_015441.2 | 54.4% | 54.4% | 1_1023del;1408T>C |
| 3 | human | 25903 | OLFML2B | olfactomedin like 2B | NM_001297713.1 | 54.4% | 54.3% | 1_1026del;1411T>C |
| 4 | human | 25903 | OLFML2B | olfactomedin like 2B | NM_001347700.1 | 54.3% | 54.2% | 1_1029del;1414T>C |
| 5 | human | 25903 | OLFML2B | olfactomedin like 2B | XM_017000967.2 | 23.4% | 19.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 63
- ORF end:
- 1290
- ORF length:
- 1227
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcca 61 ccatgaccag tgtcacccgg aggcctgcag ccacccgtca gggacacagc actgctgtga 121 caagcgacct gaacgctcgg accgcaccct ggtcctcagc actgccacag ccctcgacct 181 cagatcccag catcgccaac catgcctcag tgggaccaac actccaaaca acctcggtgt 241 ctccagatcc cacaagggag tcagtcctgc agccttctcc tcaggtacca gccaccactg 301 tggcccacac agccacccag caaccagcag ccccagctcc tccggcagtg tctcccaggg 361 aggcattgat ggaagctatg cacacagtcc cagtgcctcc caccacagtc agaacagact 421 cgctggggaa agatgctcct gctgggcggg gaacaacccc tgccagcccc acgctgagcc 481 ccgaagaaga agatgacatc cggaatgtca taggaaggtg caaggacact ctctccacaa 541 tcacggggcc gaccacccag aacacatatg ggcggaatga aggggcctgg atgaaggacc 601 ccctggccaa ggatgagcgg atttacgtaa ccaactatta ctacggcaac accctggtag 661 agttccggaa cctggagaac ttcaaacaag gtcgctggag caattcctac aagctcccgt 721 acagctggat cggcacaggc cacgtggtat acaatggcgc cttctactac aatcgcgcct 781 tcacccgcaa catcatcaag tacgacctga agcagcgcta cgtggctgcc tgggccatgc 841 tgcatgacgt ggcctacgag gaggccaccc cctggcgatg gcagggccac tcagacgtgg 901 actttgctgt ggacgagaat ggcctatggc tcatctaccc ggccctggac gatgagggct 961 tcagccagga ggtcattgtc ctgagcaagc tcaatgccgc ggacctgagc acacagaagg 1021 agaccacatg gcgcacgggg ctccggagga atttctacgg caactgcttc gtcATCTGTG 1081 GGGTGCTGTA TGCCGTGGAT AGCTACAACC AGCGGAATGC CAACATCTCC TACGCTTTCG 1141 ACACCCACAC CAACACACAG ATCGTCCCCA GGCTGCTGTT CGAGAATGAG TATTCCTATA 1201 CGACCCAGAT AGACTACAAC CCCAAGGACC GCCTGCTCTA TGCCTGGGAC AATGGCCACC 1261 AGGTCACTTA CCATGTCATC TTTGCCTACT AGGACCTAGC TTTCTTGTAC AAAGTGGTTG 1321 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1381 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 1441 ATATGCGCCT CCTCATTGTC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1501 ttgtgaaaga tt