Construct: ORF TRCN0000487887
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022012.1_s317c1
- DNA Barcode:
- CGATTGACTCAAATGCGTGCGTAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PCSK9 (255738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487887
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255738 | PCSK9 | proprotein convertase subti... | NM_174936.4 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 255738 | PCSK9 | proprotein convertase subti... | NR_110451.1 | 48% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2145
- ORF length:
- 2076
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggcaccgtc agctccaggc ggtcctggtg gccgctgcca ctgctgctgc 121 tgctgctgct gctcctgggt cccgcgggcg cccgtgcgca ggaggacgag gacggcgact 181 acgaggagct ggtgctagcc ttgcgttccg aggaggacgg cctggccgaa gcacccgagc 241 acggaaccac agccaccttc caccgctgcg ccaaggatcc gtggaggttg cctggcacct 301 acgtggtggt gctgaaggag gagacccacc tctcgcagtc agagcgcact gcccgccgcc 361 tgcaggccca ggctgcccgc cggggatacc tcaccaagat cctgcatgtc ttccatggcc 421 ttcttcctgg cttcctggtg aagatgagtg gcgacctgct ggagctggcc ttgaagttgc 481 cccatgtcga ctacatcgag gaggactcct ctgtctttgc ccatagcatc ccgtggaacc 541 tggagcggat tacccctcca cggtaccggg cggatgaata ccagcccccc gacggaggca 601 gcctggtgga ggtgtatctc ctagacacca gcatacagag tgaccaccgg gaaatcgagg 661 gcagggtcat ggtcaccgac ttcgagaatg tgcccgagga ggacgggacc cgcttccaca 721 gacaggccag caagtgtgac agtcatggca cccacctggc aggggtggtc agcggccggg 781 atgccggcgt ggccaagggt gccagcatgc gcagcctgcg cgtgctcaac tgccaaggga 841 agggcacggt tagcggcacc ctcataggcc tggagtttat tcggaaaagc cagctggtcc 901 agcctgtggg gccactggtg gtgctgctgc ccctggcggg tgggtacagc cgcgtcctca 961 acgccgcctg ccagcgcctg gcgagggctg gggtcgtgct ggtcaccgct gccggcaact 1021 tccgggacga tgcctgcctc tactccccag cctcagctcc cgaggtcatc acagttgggg 1081 ccaccaatgc ccaggaccag ccggtgaccc tggggacttt ggggaccaac tttggccgct 1141 gtgtggacct ctttgcccca ggggaggaca tcattggtgc ctccagcgac tgcagcacct 1201 gctttgtgtc acagagtggg acatcacagg ctgctgccca cgtggctggc attgcagcca 1261 tgatgctgtc tgccgagccg gagctcaccc tggccgagtt gaggcagaga ctgatccact 1321 tctctgccaa agatgtcatc aatgaggcct ggttccctga ggaccagcgg gtactgaccc 1381 ccaacctggt ggccgccctg ccccccagca cccatggggc aggttggcag ctgttttgca 1441 ggactgtgtg gtcagcacac tcggggccta cacggatggc cacagccatc gcccgctgcg 1501 ccccagatga ggagctgctg agctgctcca gtttctccag gagtgggaag cggcggggcg 1561 agcgcatgga ggcccaaggg ggcaagctgg tctgccgggc ccacaacgct tttgggggtg 1621 agggtgtcta cgccattgcc aggtgctgcc tgctacccca ggccaactgc agcgtccaca 1681 cagctccacc agctgaggcc agcatgggga cccgtgtcca ctgccaccaa cagggccacg 1741 tcctcacagg ctgcagctcc cactgggagg tggaggacct tggcacccac aagccgcctg 1801 tgctgaggcc acgaggtcag cccaaccagt gcgtgggcca caggGAGGCC AGCATCCACG 1861 CTTCCTGCTG CCATGCCCCA GGTCTGGAAT GCAAAGTCAA GGAGCATGGA ATCCCGGCCC 1921 CTCAGGAGCA GGTGACCGTG GCCTGCGAGG AGGGCTGGAC CCTGACTGGC TGCAGTGCCC 1981 TCCCTGGGAC CTCCCACGTC CTGGGGGCCT ACGCCGTAGA CAACACGTGT GTAGTCAGGA 2041 GCCGGGACGT CAGCACTACA GGCAGCACCA GCGAAGAGGC CGTGACAGCC GTTGCCATCT 2101 GCTGCCGGAG CCGGCACCTG GCGCAGGCCT CCCAGGAGCT CCAGTGAGAC CCAGCTTTCT 2161 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2221 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2281 GTGGAAAGGA CGACGATTGA CTCAAATGCG TGCGTAAACG CGTTAAGTCg acaatcaacc 2341 tctggattac aaaatttgtg aaagatt