Construct: ORF TRCN0000487888
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021648.1_s317c1
- DNA Barcode:
- CCTCCTAGCCTTTAAGTCGTATCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SPPL3 (121665)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487888
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 121665 | SPPL3 | signal peptide peptidase li... | NM_139015.5 | 99.9% | 100% | 615T>C |
| 2 | human | 121665 | SPPL3 | signal peptide peptidase li... | XM_011537925.2 | 99% | 98.4% | (many diffs) |
| 3 | mouse | 74585 | Sppl3 | signal peptide peptidase 3 | NM_029012.2 | 91.3% | 100% | (many diffs) |
| 4 | mouse | 74585 | Sppl3 | signal peptide peptidase 3 | XM_017321123.1 | 71% | 77.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1221
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcggagcag acctactcgt gggcctattc cctggtggat tccagtcaag 121 tgtctacatt tctgatttcc attcttctta tagtctatgg tagtttcagg tcccttaata 181 tggactttga aaatcaagat aaggagaaag acagtaatag ttcttctggg tctttcaatg 241 gcaacagcac caataatagc atccaaacaa ttgactctac ccaggctctg ttccttccaa 301 ttggagcatc tgtctctctt ttagtaatgt tcttcttctt tgactcagtt caagtagttt 361 ttacaatatg tacagcagtt cttgcaacga tagcttttgc ttttcttctc ctcccgatgt 421 gccagtattt aacaagaccc tgctcacctc agaacaagat ttcctttggt tgctgtggac 481 gtttcactgc tgctgagttg ctgtcattct ctctgtctgt catgctcgtc ctcatctggg 541 ttctcactgg ccattggctt ctcatggatg cactggccat gggcctctgt gtcgccatga 601 tcgcctttgt ccgcctgccg agcctcaagg tctcctgcct gcttctctca gggcttctca 661 tctatgatgt cttttgggta ttcttctcag cctacatctt caatagcaac gtcatggtga 721 aggtggccac tcagccggct gacaatcccc ttgacgttct atcccggaag ctccacctgg 781 ggcccaatgt tgggcgtgat gttcctcgcc tgtctctgcc tggaaaactg gtcttcccaa 841 gctccactgg cagccacttc tccatgttgg gcatcggaga catcgttatg cctggtctcc 901 tactatgctt tgtccTTCGC TATGACAACT ACAAAAAGCA AGCCAGTGGG GACTCCTGTG 961 GGGCCCCTGG ACCTGCCAAC ATCTCCGGGC GCATGCAGAA GGTCTCCTAC TTTCACTGCA 1021 CCCTCATCGG ATACTTTGTA GGCCTGCTCA CTGCTACTGT GGCGTCTCGC ATTCACCGGG 1081 CCGCCCAGCC CGCCCTTCTC TATTTGGTGC CATTTACTTT ATTGCCACTC CTCACGATGG 1141 CCTATTTAAA GGGCGACCTC CGGCGGATGT GGTCTGAGCC TTTCCACTCC AAGTCCAGCA 1201 GCTCCCGATT CCTGGAAGTA TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1261 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1321 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CTCCTAGCCT 1381 TTAAGTCGTA TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1441 att