Construct: ORF TRCN0000487888

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021648.1_s317c1
DNA Barcode:
CCTCCTAGCCTTTAAGTCGTATCA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
SPPL3 (121665)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487888
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 121665 SPPL3 signal peptide peptidase li... NM_139015.5 99.9% 100% 615T>C
2 human 121665 SPPL3 signal peptide peptidase li... XM_011537925.2 99% 98.4% (many diffs)
3 mouse 74585 Sppl3 signal peptide peptidase 3 NM_029012.2 91.3% 100% (many diffs)
4 mouse 74585 Sppl3 signal peptide peptidase 3 XM_017321123.1 71% 77.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1221
ORF length:
1152
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggcggagcag acctactcgt gggcctattc cctggtggat tccagtcaag
121tgtctacatt tctgatttcc attcttctta tagtctatgg tagtttcagg tcccttaata
181tggactttga aaatcaagat aaggagaaag acagtaatag ttcttctggg tctttcaatg
241gcaacagcac caataatagc atccaaacaa ttgactctac ccaggctctg ttccttccaa
301ttggagcatc tgtctctctt ttagtaatgt tcttcttctt tgactcagtt caagtagttt
361ttacaatatg tacagcagtt cttgcaacga tagcttttgc ttttcttctc ctcccgatgt
421gccagtattt aacaagaccc tgctcacctc agaacaagat ttcctttggt tgctgtggac
481gtttcactgc tgctgagttg ctgtcattct ctctgtctgt catgctcgtc ctcatctggg
541ttctcactgg ccattggctt ctcatggatg cactggccat gggcctctgt gtcgccatga
601tcgcctttgt ccgcctgccg agcctcaagg tctcctgcct gcttctctca gggcttctca
661tctatgatgt cttttgggta ttcttctcag cctacatctt caatagcaac gtcatggtga
721aggtggccac tcagccggct gacaatcccc ttgacgttct atcccggaag ctccacctgg
781ggcccaatgt tgggcgtgat gttcctcgcc tgtctctgcc tggaaaactg gtcttcccaa
841gctccactgg cagccacttc tccatgttgg gcatcggaga catcgttatg cctggtctcc
901tactatgctt tgtccTTCGC TATGACAACT ACAAAAAGCA AGCCAGTGGG GACTCCTGTG
961GGGCCCCTGG ACCTGCCAAC ATCTCCGGGC GCATGCAGAA GGTCTCCTAC TTTCACTGCA
1021CCCTCATCGG ATACTTTGTA GGCCTGCTCA CTGCTACTGT GGCGTCTCGC ATTCACCGGG
1081CCGCCCAGCC CGCCCTTCTC TATTTGGTGC CATTTACTTT ATTGCCACTC CTCACGATGG
1141CCTATTTAAA GGGCGACCTC CGGCGGATGT GGTCTGAGCC TTTCCACTCC AAGTCCAGCA
1201GCTCCCGATT CCTGGAAGTA TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1261AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1321TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CTCCTAGCCT
1381TTAAGTCGTA TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1441att

Download FASTA (ORF) (Full)