Transcript: Human NM_139015.5

Homo sapiens signal peptide peptidase like 3 (SPPL3), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
SPPL3 (121665)
Length:
4135
CDS:
492..1646

Additional Resources:

NCBI RefSeq record:
NM_139015.5
NBCI Gene record:
SPPL3 (121665)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_139015.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000296189 CTCCTCACGATGGCCTATTTA pLKO_005 1551 CDS 100% 15.000 21.000 N SPPL3 n/a
2 TRCN0000296231 GGTAGTTTCAGGTCCCTTAAT pLKO_005 582 CDS 100% 13.200 18.480 N SPPL3 n/a
3 TRCN0000073758 CCGCAGTTACAAACTAGTATA pLKO.1 2487 3UTR 100% 0.000 0.000 N SPPL3 n/a
4 TRCN0000073759 GCACCAATAATAGCATCCAAA pLKO.1 670 CDS 100% 4.950 3.960 N SPPL3 n/a
5 TRCN0000296232 GAAGCTCTTCTGTCGTTATAT pLKO_005 2115 3UTR 100% 15.000 10.500 N SPPL3 n/a
6 TRCN0000308107 GGGCATCGGAGACATCGTTAT pLKO_005 1292 CDS 100% 10.800 7.560 N SPPL3 n/a
7 TRCN0000073761 CCTGGTCTCCTACTATGCTTT pLKO.1 1314 CDS 100% 4.950 3.465 N SPPL3 n/a
8 TRCN0000307051 CCTGGTCTCCTACTATGCTTT pLKO_005 1314 CDS 100% 4.950 3.465 N SPPL3 n/a
9 TRCN0000073762 AGCACCAATAATAGCATCCAA pLKO.1 669 CDS 100% 3.000 2.100 N SPPL3 n/a
10 TRCN0000073760 CCCTTCTCTATTTGGTGCCAT pLKO.1 1516 CDS 100% 2.640 1.848 N SPPL3 n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3774 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_139015.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04756 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04756 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000475764 TGTCCGCCTGTCTCGGGATCACAC pLX_317 26.8% 100% 100% V5 n/a
4 TRCN0000487888 CCTCCTAGCCTTTAAGTCGTATCA pLX_317 27% 99.9% 100% V5 (not translated due to prior stop codon) 615T>C n/a
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